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Studies on the subcellular distribution of (Na+ + K+)-ATPase, K+-stimulated ATPase and HCO3-stimulated ATPase activities in Malpighian tubules of Locusta migratoria L


, : Studies on the subcellular distribution of (Na+ + K+)-ATPase, K+-stimulated ATPase and HCO3-stimulated ATPase activities in Malpighian tubules of Locusta migratoria L. Insect Biochemistry 21(7): 749-758

The present study confirms previous reports of the presence of (Na+ + K+)-ATPase and anion-stimulated ATPase activity in Malpighian tubules of Locusta. In addition, the presence of a K+-stimulated, ouabain-insensitive ATPase activity has been identified in microsomal fractions. Differential and sucrose density-gradient centrifugation of homogenates has been used to separate membrane fractions which are rich in mitochondria, apical membranes and basolateral membranes; as indicated by the presence of succinate dehydrogenase and the presence or absence of non-specific alkaline phosphatase activity, respectively. Relatively high specific (Na+ + K+)-ATPase activity was associated with the basolateral membrane-rich fractions with only low levels of this activity being associated with the apical membrane-rich preparation. K+-stimulated ATPase activity was also associated, predominantly, with the basolateral membrane-rich fractions. However, comparison of the distribution of this activity with that of the (Na+ + K+)-ATPase suggests that the two enzymes did not co-separate. The possibility that the K+-stimulated ATPase was not associated with the basolateral plasma membrane is discussed. Anion-stimulated ATPase activity was found in the apical and basolateral membrane-rich fractions and in the fraction containing mainly mitochondria. Nevertheless, the fact that this bicarbonate-stimulated activity did not co-separate with succinate dehydrogenase activity suggests that it was not exclusively mitochondrial in origin. These results are consistent with physiological studies indicating a basolateral (Na+ + K+)-ATPase but do not support the K+-stimulated ATPase as a candidate for the apical electrogenic pump. The possible role of the bicarbonate-stimulated ATPase activity in ion transport across both the basolateral and apical cell membranes is discussed.

US$19.90

DOI: 10.1016/0020-1790(91)90116-v


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Xie, H.; Bittar, E.E., 1994: Zinc is an inhibitor of the stimulatory response of the sodium efflux to the microinjection of cyclic AMP and forskolin in single barnacle muscle fibers. The main aim of the experiments with which this paper deals was to test the hypothesis that Zn is an inhibitor of the stimulatory response of the ouabain-insensitive component of the Na efflux to the injection of cAMP, DcAMP (dibutyryl-cAMP) and F...

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E.R.fie, M.S.; Zidan, Z.H., 1971: Effect of the rate of application and the volume of applied water on the rate of mobility of certain insecticides in clay soil. The effect of application rate and water volume on the mobility of heptachlor, disulfoton (Di-Syston) and aldicarb (Temik) in sandy and clay soils in glass tubes was determined in laboratory tests in Egypt. The results indicated that mobility was...

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