+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Studies with compounds that compete with tryptophan binding to rat hepatic nuclei

, : Studies with compounds that compete with tryptophan binding to rat hepatic nuclei. Journal of Nutrition 122(5): 1085-1095

Tryptophan has been demonstrated to affect hepatic RNA and protein metabolism. Binding of tryptophan to nuclear envelope proteins has been demonstrated to be saturable, stereospecific, and of high affinity. The hepatic nuclear envelope tryptophan binding protein (glycoprotein) has been purified to apparent homogeneity using either concanavalin A-agarose or tryptophan-agarose. The receptor has an M(r) of approximately 34,000, which is the same as that observed when [3H]tryptophan has been crosslinked to nuclear proteins. In this study, we investigated whether analogs, metabolites or related compounds of tryptophan as well as other amino acids may bind to rat hepatic nuclei using in vitro [3H]tryptophan binding assays. Our results indicate that compounds that compete with [3H]tryptophan binding to hepatic nuclei or nuclear envelopes contain the alpha-amino-propionic acid structure. Such compounds were 5-fluoro tryptophan, 7-aza tryptophan, 5-hydroxy tryptophan, alanine, phenylalanine, tyrosine, cysteine and cystine. It was of interest that, whereas tryptophan-methyl ester and tryptophan-ethyl ester competed, alpha-methyl tryptophan, N-formyl tryptophan, N-acetyl tryptophan, and N-methyl tryptophan did not compete with [3H]tryptophan binding to hepatic nuclei or nuclear envelopes. Nonetheless, only the in vivo administration of L-tryptophan was able to stimulate nucleocytoplasmic efflux of hepatic RNA and protein synthesis.


PMID: 1373445

Other references

Young, Nelson D., 1994: Analysis of an Iris hybrid zone Cline displacement for a chloroplast DNA marker in the Iris douglasiana/I innominata hybrid zone. American Journal of Botany 81(6 SUPPL ): 196

Kaiser, J., 2007: Biodefense. Proposed biosecurity review plan endorses self-regulation. Science 316(5824): 529-529

Nieto, Y.; Tu, S-M.; Bassett, R.; Jones, R.B.; Gulbis, A.M.; Tannir, N.; Kingham, A.; Ledesma, C.; Margolin, K.; Holmberg, L.; Champlin, R.; Pagliaro, L., 2016: Bevacizumab/high-dose chemotherapy with autologous stem-cell transplant for poor-risk relapsed or refractory germ-cell tumors. High-dose chemotherapy (HDC) using sequential cycles of carboplatin/etoposide is curative for relapsed germ-cell tumors (GCT). However, outcomes of high-risk patients in advanced relapse remain poor. We previously developed a new HDC regimen combi...

Mishra, D.; Juneja, M., 2015: Kocher-Debre-Semelaigne syndrome. Journal of Pediatric Neurosciences 9(3): 289-290

Hildingson, B., 1962: Nitrate as a means of suppressing faulty fermentation in cheese.. The effect of adding KNO3 to the cheese milk or whey (30 g./100 kg. cheese milk) in the manufacture of Herrgard or Svecia 45+ cheese was studied. Herrgard cheese controls had a significantly higher count of streptococci, although coliform numbers...

Collins J.M., 1982: Quantitative rates of dna synthesis during the s phase activities of dna polymerases. Federation Proceedings 41(4): ABSTRACT 5409

Bhat, K.Gopalakrishna, 2000: Robiquetia rosea Garay A new record for India. Robiquetia rosea (Lindley) Garay (Orchidaceae) is reported for the first time from India. Detailed description, illustration and relevant notes are provided.

Glaser, J.; Esser, W.; Holtmannspötter, K., 1985: Sonographic visualization of the pancreatic duct before and after secretin stimulation: an aid in the diagnosis of chronic pancreatitis. In 34 patients the diameter of Wirsung's duct was measured by real-time sonography before and after intravenous application of secretin. 25 of these patients, meeting special criteria, were chosen as normal controls; 9 patients had chronic pa...

Johnson, D.W.; Barnes, M.A.; Akers, R.M.; Pearson, R.E., 1990: Growth hormone and prolactin response to naloxone varies with season in Holstein calves. Prolactin (PRL) and somatotropin (GH) responses to single-bolus injection of 5 different doses of the lipid-soluble opioid antagonist naloxone (NAL) and the hydrophilic opioid antogonist methyl levallorphan mesilate (LMS), which does not readily c...

Nagy, K.; Young, M.; Baboonian, C.; Merson, J.; Whittle, P.; Oroszlan, S., 1994: Antiviral activity of human immunodeficiency virus type 1 protease inhibitors in a single cycle of infection: evidence for a role of protease in the early phase. The antiviral activities of two substrate-based inhibitors of human immunodeficiency virus type 1 (HIV-1) protease, UK-88,947 and Ro 31-8959, were studied in acute infections. H9 and HeLaCD4-LTR/beta-gal cells were infected either with HIV-1-IIIB...