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Analysis of population-level variation by sequencing PCR- amplified DNA


, : Analysis of population-level variation by sequencing PCR- amplified DNA. Molecular genetic analysis of populations: a practical approach: 159-187

The methodology for DNA amplification by PCR (polymerase chain reaction) and for sequencing PCR-amplified DNA is examined under the following headings: PCR machines (heating and cooling; controlling the cycle; software; effect of profile on yield and specificity of amplified products; and Taq polymerase); sample preparation (DNA extraction); designing primers; reaction conditions (additives that improve yield and specificity; contamination; and temperature cycle); cloning double-stranded PCR product; and preparing DNA template and direct sequencing (sample preparation; asymmetric PCR; biotinylated primers and magnetic beads; and sequencing double-stranded PCR product). In all, 11 protocols are presented for the various procedures covered.

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Other references

Targett N.M.; Mcconnell O.J., 1982: Detection of secondary metabolites in marine macro algae using the marsh periwinkle littorina irrorata as an indicator organism. Chemically profiled organic macroalgal extracts (24) and purified natural products (7) were bioassayed using the mesogastropod L. irrorata Say to determine if the presence of secondary metabolites in crude algal extracts could be correlated with t...

Guiguemde, W.Armand.; Shelat, A.A.; Garcia-Bustos, J.F.; Diagana, T.T.; Gamo, F-Javier.; Guy, R.Kiplin., 2012: Global phenotypic screening for antimalarials. Malaria, a devastating infectious disease caused by Plasmodium spp., leads to roughly 655,000 deaths per year, mostly of African children. To compound the problem, drug resistance has emerged to all classical antimalarials and may be emerging for...

Suchant, R., 2001: How can you measure diversity in the forest?. The use of the habitat model to determine biodiversity in the Black Forest in Germany is described, using the capercaillie (Tetrao urogallus) as indicator species for close-to-nature and structure-rich mixed mountain forests.

Humpel, C.; Ebendal, T.; Cao, Y.; Olson, L., 1993: Pentylenetetrazol seizures increase pro-nerve growth factor-like immunoreactivity in the reticular thalamic nucleus and nerve growth factor mRNA in the dentate gyrus. Neurotrophins may have a neuroprotective role and are probably involved in the control of axonal sprouting and synaptic plasticity. An antibody raised against a pro-sequence of nerve growth factor (NGF) was tested. In control undisturbed rats, a s...

Inagaki, K.; Kurosu, Y.; Sakano, M.; Sugiishi, T.; Yamamoto, G.; Noguchi, T.; Yano, H.; Izawa, H.; Hachiya, Y., 2006: Osteoporosis and periodontal disease in postmenopausal women: association and mechanisms. Many studies have attempted to define the relationship between postmenopausal osteoporosis and periodontal disease. Most studies support a positive association between these common diseases; however, many are cross-sectional in nature, include rel...

Ravel-D'-Esclapon, G.De, 1979: Industrial thermal energy service to horticulture. Agriculture (432) 295-297

Golob, T.B., 1982: Soil strength instrumentation and methodology of measurements. The design of a bevameter (Bekker value meter) that can measure soil strength values is described and illustrated with diagrams. The equipment is used at the Petawawa National Forestry Institute and can also measure the pressure/shrinkage relation...

Wollmann H.A.; Schick C.; Koch S.; Bieck P.R., 1986: Clinical pharmacological studies with phenelzine an irreversible inhibitor of monoamine oxidase. Naunyn-Schmiedeberg's Archives of Pharmacology 332(SUPPL): R97

Davydova N.N.; Rybalko A.Y.; Spiridonov M.A.; Subetto D.A., 1986: New stratigraphic data on bottom sediments of the Ladoga Lake. Pages 24-26 1986

Elonen, E.; Itala, M.; Jantunen, E.; Koistinen, P.; Koivunen, E.; Lehtinen, M.; Nousiainen, T.; Pelliniemi, T.T.; Remes, K.; Sinisalo, M.; Timonen, T.; Volin, L.; Ruutu, T., 1998: Intensive chemotherapy of acute lymphoblastic leukaemia in adults supported with G-CSF A randomized study between early and delayed start of G-CSF. British Journal of Haematology 102(1): 230, July 1