geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cryopreservation by vitrification of cultured cells and somatic embryos from mesophyll tissue of asparagus


, : Cryopreservation by vitrification of cultured cells and somatic embryos from mesophyll tissue of asparagus. Acta Horticulturae 271: 109-116

Mechanically isolated single cells of asparagus (Asparagus officinalis L.) regenerated plantlets through somatic embryogenesis. Cultured cells and embryos originated from single cells were cryopreserved by vitrification. The vitrification solution (PVS1) contains (w/v) 22% glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in 0.5M sorbitol Murashige-Skoog medium (MS). After cryoprotection with 0.5M-sorbitol MS medium containing 12% ethylene glycol, cells or embryos were exposed stepwise to 85% PVS1 at 0 degrees C. They were loaded in 0.25 ml transparent straw, and then plunged directly into liquid nitrogen. After rapid warming, PVS1 was removed and diluted stepwise. The highest survival of vitrified cells and embryos were about 65% and 50%, respectively. Survived embryos developed into plantlets.

US$29.90


Other references

Ashok Kumar Chopra, Arun Kumar Sharma, 2013: Removal of turbidity, COD and BOD from secondarily treated sewage water by electrolytic treatment. A preliminary study was conducted for the removal of turbidity (TD), chemical oxygen demand (COD) and biochemical oxygen demand (BOD) from secondarily treated sewage (STS) water through the electrolytic batch mode experiments with DC power supply...

Comerfold, J.; Dowsett, G.; Kennedy, J.; Ennis, J.; Bouchier-Hayes, D., 1986: 111 Indium labelled platelet scanning in the diagnosis of microembolic disease: a prospective study. Irish Journal of Medical Science 155(4): 121-122

Kusovac, D.; Mandic, V., 1954: Case of mole in twin pregnancy. Srpski Arhiv Za Celokupno Lekarstvo 82(6): 815-819

Burmann, Börn.M.; Uc-Mass, A.; Schweimer, K.; Gottesman, M.E.; Rösch, P., 2008: The Y39A mutation of HK022 Nun disrupts a boxB interaction but preserves termination activity. Coliphage HK022 Nun protein targets phage lambda nut boxB RNA and acts as a transcriptional terminator, counteracting the phage lambda N protein, a suppressor of transcription termination. Both Nun and N protein interact directly with RNA polymera...

Harkness, S.; Machin, S.; Waldfogel, J., 1997: Evaluating the pin money hypothesis: the relationship between women's labour market activity, family income and poverty in Britain. "In this paper we evaluate the hypothesis that the over-representation of women amongst the low paid is of little importance because women's earnings account for only a small proportion of total family income. Data from the [United Kingd...

Yaqub, T.; Shabbir, M.Zubair.; Mukhtar, N.; Tahir, Z.; Abbas, T.; Amir, E.; Defang, G., 2017: Detection of selected arboviral infections in patients with history of persistent fever in Pakistan. Surveillance is a valuable tool for understanding prevailing and previously undiagnosed infections in a geographic area. We examined 480 archived serum samples from patients with history of persistent fever (>40°C, 60-72h) who were referred to...

Kraberger, S.; Kumari, S.G.; Najar, A.; Stainton, D.; Martin, D.P.; Varsani, A., 2017: Molecular characterization of faba bean necrotic yellows viruses in Tunisia. Faba bean necrotic yellows virus (FBNYV) (genus Nanovirus; family Nanoviridae) has a genome comprising eight individually encapsidated circular single-stranded DNA components. It has frequently been found infecting faba bean (Vicia faba L.) and ch...

Fang, X-Wen.; Turner, N.C.; Xu, D-Hui.; Jin, Y.; He, J.; Li, F-Min., 2013: Limits to the height growth of Caragana korshinskii resprouts. Predawn leaf water potential (LWP), the LWP between 09:00 and 10:30 h (termed minimum LWP), stem xylem hydraulic conductivity, foliar nitrogen, leaf gas exchange and leaf traits were measured on the same days in adults and 1-year-old to 7-year-old...

Posen, S., 1957: Glycogen storage disease with unusual renal lesions; report of a case with autopsy and enzyme studies. Australasian Annals of Medicine 6(3): 238-243

Reva O.D., 1969: Phosphorylation conjugated with oxidation of different substrates in functionally and morphologically different lumbar spine areas. Ukrayins'Kyi Biokhimichnyi Zhurnal: 196