geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter


, : Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter. Plant Physiology 103(1): 97-103

Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much higher than that achieved by wounding. Northern blot analysis showed a corresponding increase in mRNA levels. After 20 h of induction of flowering plants, the response to MJ treatment was weaker in old leaves compared with young leaves, whereas the SA response was stronger in old leaves. Kinetic experiments showed that the SA response was much faster than the MJ response, suggesting that the induction mechanism of the nos promoter by these chemicals may differ. Deletion analysis showed that both SA and MJ responses require the DNA sequence between -119 and -112 from the transcription initiation site. This region contains the hexamer sequence (TGACGT) that has been found to be an important regulatory element for several promoters. The MJ response was also reduced by deletions of the CAAT box region or the sequence between -112 and -101, whereas the SA response was not significantly affected by these deletions. This suggests that the nos upstream region containing the hexamer motif is essential for the SA or MJ response and that the CAAT box region and the sequence immediately downstream from the hexamer motif are required for maximum induction by MJ.

US$19.90

PMID: 8208860

DOI: 10.1104/pp.103.1.97


Other references

Kohne, M., 1978: Recreational farm mortage and profit compensation for losses suffered by expropriation. Agrarrecht 8(4): 94-97

Peresleni, A.I.; Savvateeva, E.V.; Peresleni, I.V.; Sharagina, L.M., 1997: Mutational analysis and genetic cloning of the agnostic locus, which regulates learning ability in Drosophila. P-insertion mutations were obtained and localized by in situ methods at the agnostic gene (agn: 1-38.9; 11AB) in Drosophila. All agn mutants showed a wide spectrum of pleiotropic effects: an EMS-induced mutation of the agn-ts398 improved the abili...

Moretti, M.; Pezzani, M., 1957: Histopathological findings in the kidney of the anoxic newborn. La Pediatria 65(5): 748-763

Beckett A.H., 1986: Philosophy and practice of control of drug abuse in sport part i. Gorrod, J W , G G Gibson And M Mitchard (Ed ) Ellis Horwood Series in Biomedicine: Development Of Drugs And Modern Medicines; Conference, Chelsea, England, Uk, October 1985 X+669p Vch Publishers, Inc : New York, New York, Usa; Weinheim, West Germany; Ellis Horwood Ltd : Chichester, England, Uk (Dist By Vch Publishers: Deerfield Beach, Florida, Usa; Vch Verlagsgesellschaft: Weinheim, West Germany) Illus 563-572

Soler, Mía.José.; Riera, M.; Gutierrez, A.; Pascual, J., 2012: New options and perspectives for proteinuria management after kidney transplantation. Proteinuria has been strongly correlated with reduced function and graft survival in kidney-transplanted patients. Data regarding new strategies in proteinuria treatment and subsequent allograft survival are lacking. Similarities between chronic g...

Lemke. Gertraud., 1963: The possibility of re-using exhausted compost for mushroom growing. Further studies showed that exhausted compost can be re-used after being sterilized and enriched with cotton seed meal, the 2nd crop apparently being uninfluenced by the 1st.

Tuite, M.F., 2000: Cell biology. Sowing the protein seeds of prion propagation. Ever since Prusiner first proposed his radical "protein-only" hypothesis to explain how certain infectious proteins (prions) are transmitted from one mammal to another in the absence of DNA or RNA, scientists have been trying to prove hi...

Raffioni, S.; Miceli, C.; Vallesi, A.; Chowdhury, S.K.; Chait, B.T.; Luporini, P.; Bradshaw, R.A., 1992: Primary structure of Euplotes raikovi pheromones: comparison of five sequences of pheromones from cells with variable mating interactions. The amino acid sequences of five pheromones, Er-2, Er-3, Er-9, Er-11, and Er-20, secreted by cells of different mating types of the ciliated protozoa Euplotes raikovi, have been determined by automated Edman analyses of the whole proteins and germ...

Fairlie, W.D.; Stanton, P.G.; Hearn, M.T., 1995: Immunochemical characterization of two thyroid-stimulating hormone beta-subunit epitopes. The epitopes of human thyroid-stimulating hormone (hTSH) recognized by two murine monoclonal antibodies (MAbs), designated MAb 279 and MAb 299, have been characterized. These MAbs are highly specific for the beta-subunit of TSH. The epitope recogn...

Moullin, C., 1978: Bacteriology of the oral cavity. La Revue Francaise de la Prothese Dentaire: 36-40