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Improved procedure for a high-yield recovery of enzymatically active recombinant calf chymosin from Escherichia coli inclusion bodies


, : Improved procedure for a high-yield recovery of enzymatically active recombinant calf chymosin from Escherichia coli inclusion bodies. Protein Expression and Purification 4(1): 59-63

The high-yield recovery of enzymatically active recombinant calf chymosin from Escherichia coli inclusion bodies was achieved by optimization of solubilization and renaturation conditions. The solubilization was carried out in 8 M urea at various pHs, at various temperatures, and for various periods of time. The following values were found optimal: 1 h at 31 degrees C, pH 10.4. For successful correct refolding of solubilized prochymosin molecules it was found to be necessary to dilute the solution into an alkaline buffer (pH 10.7) in such a way that the final concentration of urea did not exceed 0.32 M and that of protein 0.275 mg/ml. Our optimized procedure gives about eight times higher yields of enzymatically active chymosin than the current published methods.

US$19.90

PMID: 8425109

DOI: 10.1006/prep.1993.1009


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Calvo Ran; Bartov Yosef, 2000: Source of salinity in the Hazeva Aquifer based on a new subdivision of the Hazeva Formation. Current Research - Geological Survey of Israel 12: 59-64

Val'-Nikov, I.; Egorov, G., 1976: Effect of sulfur containing fertilizers on the yield of crops in the middle Volga area. Agrokhimiia 100-104

Guntert M., 1985: Structure of winter groups in the nuthatch sitta pygmaea. Journal fuer Ornithologie 126(3): 346

Chen S.Z., 1993: Interpretation of rock mechanical properties by seismic waveform analysis. Eos, Transactions, American Geophysical Union: 6, Suppl., Pages 288. 1993.

Morse, RW.; Follis, TB., 1974: Physiologic normals research in lions. Unknown

Stoessl, A.J.n; Dobko, T.; Morrison, K.S.; Oakes, T.; Ho, H.H.; Snow, B.J.; Poskitt, K.J.; Dunn, H.G., 1998: Positron emission tomography studies of the dopamine system in Rett syndrome. Neurology 50(4 SUPPL 4): A7, April

Bardet, N.; Duffaud, S.; Martin, M.; Mazin, J.-Michel.; Pereda Suberbiola, X.; Vidier, J.-Pierre., 1997: Discovery of the ichthyosaur Ophthalmosaurus in the Tithonian Upper Jurassic of Boulonnais, northern France Decouverte de lichthyosaure Ophthalmosaurus dans le Tithonien Jurassique superieur du Boulonnais, Nord de la France. Neues Jahrbuch fuer Geologie und Palaeontologie Abhandlungen. Oktober; 2053: 339-354

Tríska, J.; Neuwirth, J., 1970: The so-called pulmonary alveolar proteinosis. Bratislavske Lekarske Listy 53(2): 225-229