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Molecular analysis of plants regenerated from embryogenic cultures of wheat (Triticum aestivum L.)


, : Molecular analysis of plants regenerated from embryogenic cultures of wheat (Triticum aestivum L.). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 87(7): 821-828

Total DNAs of plants regenerated from immature embryo-derived 2-month-old embryogenic calli of wheat (cultivars Florida 302, Chris, Pavon, RH770019) were probed with six maize mitochondrial genes (atpA, atp6, apt9, coxI, coxII, rrn18-rrn5), three hypervariable wheat mitochondrial clones (K', K3, X2), five random pearl millet mitochondrial clones (4A9, 4D1, 4D12, 4E1, 4E11) and the often-used wheat Nor locus probe (pTA71), in order to assess the molecular changes induced in vitro. In addition, protoplast-derived plants, and 24-month-old embryogenic and non-embryogenic calli and cell suspension cultures of Florida 302 were also analyzed. No variation was revealed by the wheat or millet mitochondrial clones. Qualitative variation was detected in the nonembryogenic suspension culture by three maize mitochondrial genes (coxI, rrn18-rrn5, atp6). A callus-specific 3.8-kb Hind III fragment was detected in all four cultivars after hybridization with the coxI gene. The organization of the Nor locus of the plants regenerated from Florida 302 and Chris was stable when compared to their respective control plants and calli. The Nor locus in regenerants of Pavon and RH, on the other hand, was found to be variable. However, Nor locus variability was not observed in 14 individual seed-derived control plants from either Pavon or RH sources. In Pavon, a 3.6-kb Taq I or a 5.6-kb Bam HI+ Eco RI fragment was lost after regeneration. In one of the RH regenerants, which lost a fragment, an additional fragment was observed.

US$19.90

PMID: 24190468

DOI: 10.1007/BF00221134


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Bhattacharjee, Y., 2008: Nonproliferation. Dutch revise policy blocking Iranian students. Science 319(5863): 556-556

McMillin, B.A.; Jasmund, J.M., 1985: A quality assurance study of height and weight measurements. The QA representatives of the nursing and dietetics departments were able to work together toward resolving a shared QA concern. The problems associated with height and weight measurement were studied comprehensively. On the basis of the initial s...

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Biardeau, X.; Biardeau, X.; Lam van Ba, O.; Caremel, R.; Aharony, S.; Lotouchin, O.; Barbe, M.; Tuite, G.; Jacques, L.; Ruggieri, M.; Campeau, L.; Corcos, J., 2015: Not Available. Progres en Urologie 25(13): 847-847

Southwell, CJ., 1984: Variability in grouping in the eastern grey kangaroo, Macropus giganteus. 2. Dynamics of group formation. The effects of season, population density and habitat on aspects of group formation in the eastern gray kangaroo were examined. The rate at which groups join and split increased exponentially with population density, but did not vary with season o...

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