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Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction


, : Proteinase inhibition of the detection of Listeria monocytogenes in milk using the polymerase chain reaction. Letters in Applied Microbiology 18(1): 59-61

The direct detection, using the polymerase chain reaction (PCR), of Listeria monocytogenes added to cows' milk was inhibited at some milk concentrations. This inhibitor was moderately heat-stable. Inhibition could be prevented by the addition of Bovine Serum Albumin (BSA) or proteinase inhibitors to the PCR and the evidence suggests that the inhibitor was plasmin.

US$19.90

DOI: 10.1111/j.1472-765x.1994.tb00802.x


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