geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A simple technique to pretreat urine and serum samples for quantitation of schistosome circulating anodic and cathodic antigen


, : A simple technique to pretreat urine and serum samples for quantitation of schistosome circulating anodic and cathodic antigen. Acta Tropica 56(1): 55-63

For the detection of the circulating schistosome antigens CAA (circulating anodic antigen) and CCA (circulating cathodic antigen) in serum and urine samples of Schistosoma infected individuals, pretreatment of samples with trichloroacetic acid (TCA) is a standard procedure. In the present study several methods were evaluated in order to develop a more simple and rapid technique than the - especially for pretreatment of urine samples - laborious TCA technique. Optimal results were obtained with a method in which serum or urine samples were pretreated by a heat-incubation step (70 degree C, 30 min) in an alkaline buffer (pH 9.6). In a comparison of the new technique with the TCA pretreatment, serum and urine samples of S. mansoni infected individuals from Zaire (n=80) and of uninfected controls from The Netherlands (n=208) were pretreated and assayed for CAA and CCA. Both pretreatment techniques showed similar sensitivities and specificities for CAA and CCA in serum and CCA in urine. However, for the determination of CAA in urine the new technique performed significantly better, resulting in an increase of the sensitivity from 32 to 70% (titre determination).

US$19.90

PMID: 8203296

DOI: 10.1016/0001-706x(94)90040-x


Other references

Bednar, B.; Jirasek, A.; Stejskal, J.; Chytil, M., 1961: Secondary uremic oxalosis. Casopis Lekaru Ceskych 100: 705-708

Anonymous, 2011: The star that should not exist. A team of European astronomers has used ESO's Very Large Telescope (VLT) to track down a star in the Milky Way that many thought was impossible. They discovered that this star is composed almost entirely of hydrogen and helium, placing it in...

Weiss G., 1976: Child psycho pharmacology. Siva Sankar, Dd. . Psychopharmacology Of Childhood. 381p. Illus. Pjd Publications Limited: Westbury, N.y., Usa. Isbn 0-915340-00-3. 123-137

Crozier,R.H.; Schluns,H., 2008: Genetic caste determination in termites out of the shade but not from Mars. Several ant species are known with genetic effects on caste determination but, for termites, the role of environment has been assumed to be omnipotent. Now Hayashi et al. report that commitment to the nymph and worker pathways in <i>Reticuli...

Shiba, S.; Nishida, Y.; Park, Y.S.; Lijima, S.; Kobayashi, T., 1994: Improvement of cloned alpha-amylase gene expression in fed-batch culture of recombinant Saccharomyces cerevisiae by regulating both glucose and ethanol concentrations using a fuzzy controller. The effect of ethanol concentration on cloned gene expression in recombinant Saccharomyces cerevisiae strain 20B-12 containing one of two plasmids, pNA3 and pNA7, was investigated in batch cultures. Plasmids pNA3 and pNA7 contain the alpha-amylase...

Bindu, G.Hima.; Trivedi, R.; Kashyap, V.K., 2005: Genotypic polymorphisms at seventeen autosomal short tandem repeat loci in four tribal populations of Andhra Pradesh, India. Four tribal populations of Andhra Pradesh, South India (1), Chenchu (n=100), Lambadi (n=107), Naikpod Gond (n=104) and Yerukula (n=101) were analyzed for DNA polymorphisms at 15 tetranucleotide and 2 pentanucleotide short tandem repeat (STR) loci...

Richards, F.O.; Eigege, A.; Pam, D.; Kal, A.; Lenhart, A.; Oneyka, J.O.A.; Jinadu, M.Y.; Miri, E.S., 2005: Mass ivermectin treatment for onchocerciasis: lack of evidence for collateral impact on transmission of Wuchereria bancrofti in areas of co-endemicity. There has long been interest in determining if mass ivermectin administration for onchocerciasis has 'unknowingly' interrupted lymphatic filariasis (LF) transmission where the endemicity of the two diseases' overlaps. We studied 11...

Anonymous, 1994: Utilization management checklist. Health System Leader 1(7): 16-17

Buschmann, R.J.; Manke, D.J., 1981: Morphometric analysis of the membranes and organelles of small intestinal enterocytes 1. fasted hamster mesocricetus auratus auratus. A comprehensive morphometric analysis of the absorptive enterocyte of the fasted hamster small intestine was carried out. By sterologic methods, the volume density of the organelles, the surface density of the membranes and the numerical density o...

Tan, A.Y.J.; Macachor, J.D.; Hee, H.Ing., 2017: Managing the marginally raised aPTT-looking beyond the absolute number. Paediatric Anaesthesia 27(7): 779-780