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Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed

, : Assessment of microinjection for introducing DNA into uninuclear microspores of rapeseed. Plant Cell, Tissue and Organ Culture 40(1): 97-100

Approximately 2,000 embryogenic uninuclear microspores of rapeseed (Brassica napus) cv. Topas were intranuclearly injected with a chimaeric beta-glucuronidase (Escherichia coli Uid A) gene. Stable integration had not occurred among 55 plants that were regenerated. Coinjection of the dye Lucifer Yellow and detection of injected DNA by the polymerase chain reaction revealed high frequencies of transfer. However, the amount of DNA injected was less than 20 copies, which may have been insufficient for stable transformation of microspores.


DOI: 10.1007/bf00041124

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