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Comparison of the nuclear inclusion b protein and coat protein genes of five papaya ringspot virus strains distinct in geographic origin and pathogenicity


, : Comparison of the nuclear inclusion b protein and coat protein genes of five papaya ringspot virus strains distinct in geographic origin and pathogenicity. Phytopathology 84(10): 1205-1210

A complementary DNA library was constructed to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residues and represented part of the nuclear inclusion a (NIa) gene, the complete nuclear inclusion b (NIb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the nonpapaya-infecting Florida strain, PRSV W(fl) (W(fl)), and the nonpapaya-infecting Australia strain PRSV W(aust) (W(aust)). The NIb region had 83.1% nucleotide identity and 95.7% amino acid identity compared to that of HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similar trend also was found when the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1 and PRSV W(fl), with 84.7 and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acid identity (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble bases and, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the same protein conformation. The CP region showed 89.6-90.3% nucleotide identities and 93.2-94.8% amino acid identities with the compared strains and the 3' noncoding region showed 91.4 and 96.4% identities. Analysis of the N terminus ol YK CP supported the cleavage site as previously predicted, VYHE/SRGT; however, a possible alternative site at VFHQ/SKNE, 20 amino acids downstream, also was present. The different amino acid residues among the CP of PRSV strains were located mostly at the N-terminal regions of the CP. Alignment of the CP of five sequenced PRSV strains indicated that the variation followed the differences in geographic origins rather than host specificity.

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Alukhanian, O.A.; Abramov, I.G.; Korochanskaia, S.P., 2007: Indices of antioxidant system of blood in differential diagnosis of obliterating atherosclerosis and obliterating thromboangiitis of the lower limbs. The work was aimed at elucidating the role of the indices of the antioxidant system (AOS) of the blood and nitrogen oxide content in differential diagnosis of atherosclerosis obiiterans (AO) and thromboangiitis obiiterans (TO) of the lower extremi...

Wood I.B., 1991: The Mount Lyell Mining and Railway Company Limited: an environmental case study. History of the mining operation, the resultant environmental impacts and associated issues and lessons.

Newman, James E., 1962: Some aspects of applied meteorology in the soils and crop sciences. The central concern of applied meteorology in soil and crop sciences is the fate of energy. This involves the need for complete understanding as to how energy is transmitted, stored, and dissipated within soil profiles, in vegetative covers, as we...

Dickens G., 1989: George Renison Bell. Newsletter - Tasmania, Department of Mines 23: 23-24

Agrawala N.; Dixit R.K., 1979: Seasonal variations in the pharyngeal and pronephric thyroid tissues of the fresh water teleost puntius sophore. Pharyngeal and pronephric thyroid follicle activity was assessed histologically by the study of size of thyroid follicles, the shape and cell height of epithelial cells and the nature of their colloid. These follicles exhibited notable seasonal ch...

Colloff, M.J.; Howe, C.W.; McSharry, C.; Smith, H.V., 1992: Characterization of IgE antibody binding profiles of sera from patients with atopic dermatitis to allergens of the domestic mites Dermatophagoides pteronyssinus and Euroglyphus maynei, using enhanced chemiluminescent immunoblotting. A rapid, sensitive, chemiluminescent immunoblotting method was used to identify IgE-binding components of Dermatophagoides pteronyssinus and Euroglyphus maynei that were recognized by sera of 16 D. pteronyssinus-sensitive patients with atopic derm...

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Goldstone A.H.; Anderson C.C.; Linch D.C.; Franklin I.M.; Boughton B.J.; Cawley J.C.; Richards J.D.M., 1986: Autologous bone marrow transplantation following high dose chemotherapy for the treatment of adult patients with acute myeloid leukemia. Adult patients [24] with acute myeloid leukaemia (AML) were treated with intensive chemotherapy followed by autologous marrow rescue. The procedure was repeated twice in eight patients. Eleven of 16 patients treated in first remission continue in...

Kubota Y.; Funaki J.; Tanaka M.; Sasaki H., 1989: Electron microscope histochemistry of lectin binding sites in 1 2 dimethylhydrazine induced colon cancer in mice. 1,2-Dimethylhydrazine certainly induced colon cancer in mice and its histology demonstrated moderate to well differentiated adenocarcinoma. We divided carcinogenesis into three steps based on CHANG's classification. Using light and electron m...

Murie, R.; Diehl, H., 1954: Effects of heating vitamin B12 in a stream of dry nitrogen at various temperatures. At 180[degree]C, in an atmosphere of N2, 1 molecule of ammonia is expelled from vitamin B12; the process is accompanied by a much greater loss in weight than would be expected for expulsion of this ammonia alone. The additional loss may be merely...