geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Development and optimisation of an immunoaffinity-based solid-phase extraction for chlortoluron


, : Development and optimisation of an immunoaffinity-based solid-phase extraction for chlortoluron. Journal of Chromatography A 697(1-2): 131-136

The determination of trace organics such as pesticides in biological and environmental samples require rapid, easy-to-use, reliable sample preparation procedures. Solid-phase extraction using silica or bonded silicas has proven useful for broad-range screening. We have used antisera to chlortoluron immobilised onto silica as a "tailor-made" solid-phase extraction system. The chlortoluron can be selectively retained and eluted using a simple phosphate buffered saline/ethanol mixture. Preliminary studies have demonstrated that the immuno column has a high volume breakthrough (at least 11 of water) and can retain up to 500 ng of chlortoluron. Quantitative recovery from tap water, river water, drinking water, plasma and urine is achieved along with an HPLC trace free from co-eluting compounds at the chlortoluron retention time.

US$19.90

DOI: 10.1016/0021-9673(94)01088-v


Other references

Breiman, R.F., 2001: Vaccines as tools for advancing more than public health: perspectives of a former director of the National Vaccine Program office. With application of new technologies and licensing of new vaccines, vaccines will soon be better by several orders of magnitude. Yet barriers impede the introduction and wide use of new vaccines. Most of the diseases for which we will have vaccine...

Zhou QiXing; Dai LiMei; Bell, R.W., 1995: An integrated planning for reuse and wetland strategy of waste water from rural enterprises: a case study. A waste water reuse engineering was designed and then operated in Hongshan, a small town in Zhejiang Province, China, in order to solve pollution and shortage of water resources due to the development of rural enterprises. The results showed that...

Levy, M.; Levin, D.A., 1975: Genic Heterozygosity and Variation in Permanent Translocation Heterozygotes of the OENOTHERA BIENNIS Complex. Genic heterozygosity and variation were studied in the permanent translocation heterozygotes Oenothera biennis I, Oe. biennis II, Oe. biennis III, Oe. strigosa, Oe. parviflora I, Oe. parviflora II, and in the related bivalent formers Oe. argillico...

Lewis, R.A.; Buss, D.D., 2001: Outpatient shoulder surgery: a prospective analysis of a perioperative protocol. During a 10-month period, 106 consecutive outpatient shoulder procedures were done in 102 patients who were followed up prospectively for a minimum of 6 months. The procedures included arthroscopic surgery alone (60%) and open surgeries with or wi...

Demarco, C.T., 1976: Breach of duty. When are you liable for drug-related injuries?. Nursing 6(4): 103-4, 106

Fulda, S., 2008: Modulation of TRAIL-induced apoptosis by HDAC inhibitors. Triggering apoptosis, the cell's intrinsic death program, is a promising approach for cancer therapy. TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily of death inducing ligands, is of special interest for cancer...

Kuhn, H.; Bräunlich, J.; Hammerschmidt, S.; Wirtz, H., 2004: Candidate genes upregulated in density dependent growth inhibition of lung cancer cells. Prognosis of lung cancer remains poor despite the recent development of new chemotherapeutic agents. Novel therapeutic strategies therefore need to be developed. The search for factors inhibiting tumor growth in a paracrine/autocrine fashion might...

Domin W.S.; Chait A.; Deeb S.S., 1991: Transcriptional activation of the lipoprotein lipase gene in macrophages by dexamethasone. The effect of dexamethasone on lipoprotein lipase (LPL) gene expression during macrophage differentiation was investigated by using the human monocytic leukemia cell line THP-1 and human monocyte-derived macrophages. Addition of dexamethasone to T...

Kerambrun, E.; Henry, F.; Rabhi, K.; Amara, R., 2015: Effects of chemical stress and food limitation on the energy reserves and growth of turbot, Scophthalmus maximus. The objective of the present study is to examine the growth and energetic performance of juvenile turbot after exposure to contaminated sediment and during the subsequent recovery period with or without food limitation. We designed a two-step expe...

Seol, S.Y.ng; Kim, N.H.e; Shin, H.S.op; Lee, Y.C.ul; Cho, D.T.ek, 1999: Molecular genetic characteristics of trimethoprim resistance in clinical and normal fecal isolates of Escherichia coli. One hundred and thirty trimethoprim-resistant R plasmids derived from of Escherichia coli isolated from clinical specimens and feces of healthy collegians were examined for incompatibility, EcoRI endonuclease restriction fragment pattern, and Sout...