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Enzymes as silage additives for grass-clover mixtures


, : Enzymes as silage additives for grass-clover mixtures. Grass & Forage Science 49(3): 305-315

Seven laboratory-scale experiments were carried out to study the effects of cellulases/hemicellulases on silage fermentation of herbage from mixed swards of timothy (Phleum pratense), meadow fescue (Festuca pratensis) and red clover (Trifolium pratense). Enzyme-treated silage (approximately 3500 HEC units kg-1 grass) reached a low pH sooner, had lower end pH, contained less NH-3-N and more tactic acid than did the untreated silage (P lt 0.05). Applied with an inoculant, these effects were even stronger. With easily ensiled crops (experiments 1, 2, 5 and 6) preservation was first of all improved by inoculation of lactic acid bacteria; however, for the low-sugar crops (experiment 3, 4 and 7) enzyme treatment was more significant. The enzymes derived from Aspergillus spp. gave more reesei. The Trichoderma enzymes liberated 4.8 g WSC kg-1 FM gamma-irradiated grass during 60 d at pH 4.3 (P lt 0.05). On average, for all silages enzyme treatment increased the sum of residual sugar and fermentation products by 3.7 g kg-1 FM (21 g kg-1 DM) compared with the silage, not treated with enzymes (P lt 0.001). Enzyme treatment increased the instantly degradable part of the feed, but total in sacco and in vitro digestibilities were not affected.

US$19.90

DOI: 10.1111/j.1365-2494.1994.tb02005.x


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