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In vivo metabolism of pentachlorophenol and aniline in Arctic charr (Salvelinus alpinus L.) larvae


, : In vivo metabolism of pentachlorophenol and aniline in Arctic charr (Salvelinus alpinus L.) larvae. Bulletin of Environmental Contamination and Toxicology 54(5): 711-716

S. alpinus larvae were divided into 8 groups of 10 fish and placed in 50 ml disposable tubes containing 20 ml of water at 6 degrees C. 14C-pentachlorophenol (PCP) at 0.6 micro g (in 10 micro l of ethanol) was added to four of the tubes and 14C-aniline at 2 micro g (in 10 micro l of ethanol) was added to the remaining 4 tubes. After exposure for 48 h the larve were removed from the medium, killed, frozen at -20 degrees C and stored for approx. 1 month at this temp. No mortality was found to occur during the experiment, and neither the confinement nor the presence of pollutants were observed to stress the larvae. After 2 d of exposure the parent compound, aniline, accounted for less than 15% of the total 14C found in the water, indicating extensive metabolism of the chemical. In the case of PCP, approx. 19% of the 14C dissolved in water was associated with the parent compound. At the end of the 48-h period of exposure to aniline, 12.9% of the radioactivity was found to be present in the larvae and 66.1% was dissolved in the water; the remaining 22.1% was unaccounted for and it was presumed that the loss was due to volatilization. The radioactivity following the PCP tests was found to be concentrated into the larvae and no losses were recorded after 48 h. Exposure to PCP resulted in the formation of PCP-glucuronide and PCP-sulfate in the larvae, but there was no trace of chlorinated hydroquinone. The main metabolic pathway for aniline was found to be N-acetylation, since after 48-h exposure more than 75% of the total radioactivity was found as acetanilide; 4-aminophenol, a minor metabolite, was also identified. It is concluded that S. alpinus larvae are able to biotransform chemicals during early life stages by several metabolic reactions.

US$19.90

PMID: 7780214

DOI: 10.1007/bf00206103


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