+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In vivo metabolism of pentachlorophenol and aniline in Arctic charr (Salvelinus alpinus L.) larvae

, : In vivo metabolism of pentachlorophenol and aniline in Arctic charr (Salvelinus alpinus L.) larvae. Bulletin of Environmental Contamination and Toxicology 54(5): 711-716

S. alpinus larvae were divided into 8 groups of 10 fish and placed in 50 ml disposable tubes containing 20 ml of water at 6 degrees C. 14C-pentachlorophenol (PCP) at 0.6 micro g (in 10 micro l of ethanol) was added to four of the tubes and 14C-aniline at 2 micro g (in 10 micro l of ethanol) was added to the remaining 4 tubes. After exposure for 48 h the larve were removed from the medium, killed, frozen at -20 degrees C and stored for approx. 1 month at this temp. No mortality was found to occur during the experiment, and neither the confinement nor the presence of pollutants were observed to stress the larvae. After 2 d of exposure the parent compound, aniline, accounted for less than 15% of the total 14C found in the water, indicating extensive metabolism of the chemical. In the case of PCP, approx. 19% of the 14C dissolved in water was associated with the parent compound. At the end of the 48-h period of exposure to aniline, 12.9% of the radioactivity was found to be present in the larvae and 66.1% was dissolved in the water; the remaining 22.1% was unaccounted for and it was presumed that the loss was due to volatilization. The radioactivity following the PCP tests was found to be concentrated into the larvae and no losses were recorded after 48 h. Exposure to PCP resulted in the formation of PCP-glucuronide and PCP-sulfate in the larvae, but there was no trace of chlorinated hydroquinone. The main metabolic pathway for aniline was found to be N-acetylation, since after 48-h exposure more than 75% of the total radioactivity was found as acetanilide; 4-aminophenol, a minor metabolite, was also identified. It is concluded that S. alpinus larvae are able to biotransform chemicals during early life stages by several metabolic reactions.


PMID: 7780214

DOI: 10.1007/bf00206103

Other references

Leonov, YuA.; Kharitonova, NN., 1972: The role of Microtus oeconomus in epizootiology of Omsk haemorrhagic fever. Unknown

Tarik, E.; Ahmet, T.; Dogan, A.; Mustafa, A.; Cavit, O., 2000: Metabolic risk factors and saturation of urine in cases with first-time and recurrent calcium oxalate urinary stone formation in Turkey. European Urology 37(Suppl 2): 30, March

Abanades, Juan Carlos, 2002: The maximum capture efficiency of CO2 using a carbonation/calcination cycle of CaO/CaCO3. The use of natural calcium carbonates as regenerable CO2 sorbents in industrial processes is limited by the rapid decay of the carbonation conversion with the number of cycles carbonation/calcination. However, new processes are emerging to capture...

Cook, G.C., 1986: The clinical significance of gastrointestinal helminths--a review. Gastrointestinal helminths (nematodes, trematodes and cestodes) constitute some of the most common and important infective agents of mankind and are responsible for much morbidity and some mortality. Whereas many symptoms and signs are confined to...

Cocchi D.; Fraschini F.; Jalanbo H.; Muller E.E., 1974: Role of brain catecholamines in the post castration rise in plasma luteinizing hormone of pre puberal rats. Endocrinology 95(6): 1649-1657

Dixit V.P.; Gupta R.S., 1984: Effects of dihydropalmitinium hydroxide isolated from the roots of berberis chitria on intact spayed estradiol dipropionate pregnant female gerbils meriones hurrianae. Ethanolic extract of the defatted roots of B. chitria was chromatographed when a compound-I (C21H25O5N) characterized as dihydropalmitinium hydroxide [DPH] was isolated. Chronic administration of DPH for 20 days reduces the weights of ovary, uteru...

Schmoll, E.; Alvarez, W.C., 1911: Intestinal Indigestion in Adults. California State Journal of Medicine 9(8): 313-315

Mio, K.; Stern, R., 2002: Inhibitors of the hyaluronidases. The inhibitors of hyaluronidase present in mammalian sera, first described half a century ago, have remained uncharacterized. Because of increased interest in hyaluronidases and their hyaluronan substrate, a study of these inhibitors was undertake...

Irving, C.S.; Lifschitz, C.H.; Marks, L.M.; Nichols, B.L.; Klein, P.D., 1986: Polyethylene glycol polymers of low molecular weight as probes of intestinal permeability. I. Innovations in analysis and quantitation. The differential passive permeation of polymers of different lengths across the intestinal mucosa has been proposed as a probe to test mucosal integrity under a variety of physiologic and pathologic conditions. Polyethylene glycol (PEG) 400 contai...

Bossard, M.J.; Tomaszek, T.A.; Thompson, S.K.; Amegadzie, B.Y.; Hanning, C.R.; Jones, C.; Kurdyla, J.T.; McNulty, D.E.; Drake, F.H.; Gowen, M.; Levy, M.A., 1996: Proteolytic activity of human osteoclast cathepsin K. Expression, purification, activation, and substrate identification. Human cathepsin K is a recently identified protein with high primary sequence homology to members of the papain cysteine protease superfamily including cathepsins S, L, and B and is selectively expressed in osteoclasts (Drake, F. H., Dodds, R., Ja...