+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interference with superinfection and with cell killing and determination of host range and growth kinetics mediated by feline leukemia virus surface glycoproteins

, : Interference with superinfection and with cell killing and determination of host range and growth kinetics mediated by feline leukemia virus surface glycoproteins. Journal of Virology 67(7): 4142-4153

The functions of the surface glycoproteins (SU) of feline leukemia viruses (FeLVs) are of interest since these proteins mediate virus infection and interference and are critical determinants of disease specificity. In this study, we examined the biochemical and genetic determinants of SU important to virus entry and cell killing. In particular, we developed and used vesicular stomatitis virus (VSV)/FeLV pseudotype virus interference assays to determine interference subgroupings and assess mechanisms of host cell restriction. We also assessed roles of SU in virus growth kinetics and in the inhibition of cell killing caused by superinfection with cytopathic virus. Subgroup classification by VSV/FeLV pseudotype assay was in agreement with that defined previously by focus interference assay and was found to be determined by changes near the N terminus of SU for FeLV subgroups A (FeLV-A) and C. Virus host range restriction was found to be mediated at the level of virus entry in most cases, although postentry events mediated restriction in the failure of a subgroup A-like, T-cell cytopathic and immunodeficiency-inducing clone (FeLV-FAIDS-EECC) to replicate in feline fibroblasts. FeLV-FAIDS-EECC-induced cell killing was also inhibited by prior infection with one of two FeLV-A isolates. This inhibition could be conveyed by as few as four amino acid changes near the N terminus of the FeLV-A SU and also appeared to be mediated at a postentry level. Lastly, the SU-coding sequence was also found to determine differences in growth kinetics of viruses within the same subgroup. These studies demonstrate that subtle alterations in the FeLV SU, particularly in the N-terminal region, impart multiple significant functional differences which distinguish virus variants.


PMID: 8389921

Other references

Godfrey, K., 1997: Trouble on tap. Nursing Times 93(13): 16-17

Cortazal, M.; Larson, E.L., 2010: The medical literature and the discipline of infection control. Journal of Infection and Public Health 2(2): 53-54

Gordon, J.B.; Pemberton, S.G.; Gingras, M.K.; Konhauser, K.O., 2008: Biogenically enhanced permeability; a petrographic analysis of Macaronichnus segregatus burrowed Bluesky and Cardium Formation sandstones. Early diagenesis of siliciclastic sands in marginal-marine environments begins immediately upon deposition. Moreover, some marginal-marine settings support large populations of burrowing macrofauna animals, which can have a significant effect on e...

Murphy, M.J.; D'Alessandro, D.M.; Kepert, C.J., 2014: A porous Mn(V) coordination framework with PtS topology: assessment of the influence of a terminal nitride on CO2 sorption. A new coordination framework material, [Zn{MnN(CN)4(H2O)}]·2H2O·MeOH, has been characterised crystallographically and the effect of a terminal nitride on the N2, H2 and CO2 sorption capacities of the material assessed through porosimetery measur...

Brand, A.H.; Scurry, J.P.; Planner, R.S.; Grant, P.T., 1995: Grapelike leiomyoma of the uterus. A 24-year-old nulliparous woman underwent laparotomy for a large pelvic mass. Grapelike tumor extending from the uterus into the broad ligaments and peritoneal cavity was found. A diagnosis of sarcoma appeared likely, but radical surgery was avoid...

Bradley, M.; Ramirez, I.; Cheung, K.; Gholkar, A.A.; Torres, J.Z., 2017: Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks. Multi-protein complexes, rather than single proteins acting in isolation, often govern molecular pathways regulating cellular homeostasis. Based on this principle, the purification of critical proteins required for the functioning of these pathway...

Wadehra, M.; Iyer, R.; Goodglick, L.; Braun, J., 2001: The role of epithelial membrane protein-2 in the trafficking of beta1 integrin. Integrins are a family of surface receptors used by cells to recognize and respond to their environment. Our laboratory has recently identified a protein that may help modulate integrin expression. Epithelial Membrane Protein-2 (EMP2) is a four tr...

Sharma, J.; Imae, T., 2009: Recent advances in fabrication of anisotropic metallic nanostructures. The current review describes a critical survey of various methods for the shape- and size-selective synthesis of anisotropic metallic nanostructures, especially, of gold and silver. Although substantial progress in the size and shape control of me...

Banks, RC.; Clench, MH.; Barlow, JC., 1973: Bird collections in the United States and Canada. Auk, 901: 136-170

Struthers, R.; Hodge, F.Schanche.; Geishirt-Cantrell, B.; D.C.ra, L., 2003: Participant experiences of Talking Circles on type 2 diabetes in two Northern Plains American Indian Tribes. The Talking Circle, a culturally appropriate, 12-week educational intervention, was employed on two Northern Plains American Indian reservations to provide information on type 2 diabetes. In a phenomenological study, funded as a minority supplemen...