+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Rapid metabolism of isoflavonoids in elicitor-treated cell suspension cultures of Pueraria lobata

, : Rapid metabolism of isoflavonoids in elicitor-treated cell suspension cultures of Pueraria lobata. Phytochemistry (Oxford) 38(2): 373-380

Upon treatment with yeast extract as an elicitor to cell suspension cultures of Pueraria lobata, the constitutive isoflavonoid conjugates (7-O-glucoside-6"-O-malanyl esters) showed a rapid decline in their contents within 4 hr, followed by both reaccumulation of the conjugates and formation of isoflavones. Simultaneous treatment of a potent translation inhibitor, cycloheximide (200 mu-M), could not suppress the elicitor-inducible decrease of the preexisting isoflavonoids, although it resulted in almost complete inhibition of both reaccumulation of the conjugates and formation of isoflavones. Exogenously applied 14C-labelled isoflavones were readily converted to their respective malonyl glucosides in the cells. When yeast extract was applied to the cell cultures in which (14C)-daidzein had been incorporated into its malonyl glucoside, the methanol-soluble radioactivity decreased rapidly within 4 hr and it was not recovered for 48 hr, indicating that most of the conjugate was incorporated into an insoluble cell wall fraction. The rapid response upon elicitation was selective for isoflavonoids and not for flavonoids.


DOI: 10.1016/0031-9422(94)00640-f

Other references

Greenhouse, L., 2005: Justices explore U.S. authority over states in assisted suicide. New York Times on the Web: A1, A32-A1, A32

Doermann, A.H.; Hill, M.B., 1952: The arrangement of genetic loci in bacteriophage T4. An abstract.

Dhatariya, K.K., 2006: Policing of information from internet breast cancer list: findings may not be generally applicable. Bmj 332(7549): 1095-1095

Duek, H.; Decourt, J.R., 1953: Forceps injuries. Obstetricia Y Ginecologia Latino-Americanas 11(10): 413-422

Hara, K., 2001: Report on Options for the Control of Influenza Virus IV. Uirusu 50(2): 331-336

Mathews, H.L.; Witek Janusek, L., 2002: Host defense against oral, esophageal, and gastrointestinal candidiasis. Calderone, Richard A [Author] [Candida and candidiasis] Candida and candidiasis 179-192

Page, J.M.; Schneeweiss, S.; Whyte, H.E.; Harvey, P., 1993: Ocular sequelae in premature infants. Objective. The authors report on the incidence of myopia and strabismus at 12 and 24 months postterm in a cohort of 190 premature infants with birth weights of less than 1251 g born in 1986 and 1987. Methods. The neonatal and follow-up eye charts...

Bosch, T.J.; Maslam, S.; Roberts, B.L., 1995: A polyclonal antibody against mammalian FOS can be used as a cytoplasmic neuronal activity marker in a teleost fish. A polyclonal antibody raised against a conserved region of a mammalian FOS sequence was tested for its use as an activity marker in the rainbow trout. The FOS-like expression in the trout is entirely cytoplasmic and appears in a Nissl-like pattern...

Protoplasma, 1967: Symposium on molecular organization of unit membrane june 15 18 1965. Protoplasma 63(1-3): 147-181

Norman, J.A.; Schmidt, K.W.; Grow, J.B., 1950: Congenital arteriovenous fistula of the cervical vertebral vessels with heart failure in an infant. Journal of Pediatrics 36(5): 598-604