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Re-evaluation of malondialdehyde and thiobarbituric acid-reactive substances as indices of autoxidation based on oxygen consumption

, : Re-evaluation of malondialdehyde and thiobarbituric acid-reactive substances as indices of autoxidation based on oxygen consumption. Journal of Agricultural & Food Chemistry 41(1): 1-4

Soybean oil was oxidized at 170 and 40 degree C in a closed vessel to measure the oxygen consumption, which is assumed to be the most reliable index for the evaluation of autoxidation. It was demonstrated that the formation of malondialdehyde (MDA) and thiobarbituric acid (TBA)-reactive substances correlated linearly with the consumed oxygen until the latter value reached 500 mu-mol/L at 170 degree C. When the oxygen consumption exceeded the value, MDA and TBA-reactive substances did not increase but rather tended to decrease. On the other hand, in the autoxidation of the oil at 40 degree C using 2,2'-azobis(2,4-dimethylvaleronitrile) as an initiator, MDA and TBA-reactive substances increased linearly with the oxygen consumption at least until the latter reached 1500 mu-mol/L.


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