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Selection, enrichment and initial characterization of an elongated cell culture of tobacco


, : Selection, enrichment and initial characterization of an elongated cell culture of tobacco. Plant Science (Limerick) 103(1): 73-79

When tobacco NT-1 (Nicotiana tabacum L. cv. Bright Yellow) cells were grown under stationary conditions in the dark, a subset of the cells formed linear chains, each cell being approximately 2-5 times more elongated along one axis. When enriched cultures of the elongated cells (NT-E) were subcultured on the shaker, no reversion to the nearly isodiametric NT-1 phenotype occurred. During the 15-day culture cycle, final cell titer of NT-1 exceeded those of NT-E cells in both shaker and stationary culture conditions by a factor of 1.6 and 2.4, respectively. Length/width ratios of NT-1 cells cultured under stationary conditions were constant (average, 1.5) during the 2-week culture period, whereas length/width ratios of NT-E cells subcultured under identical conditions increased from 2.5 to 3.5 over the 2-week culture period. One-dimensional SDS-PAGE protein profiles for NT-E cells maintained as stationary or suspension cultures were very similar, but were quantitatively and qualitatively different relative to the protein profile of NT-1 cells grown in suspension. The differences between the progenitor (NT-1) and elongated (NT-E) cell types documented in this study indicate that the NT-E cell type is the result of a terminal differentiation event of the NT-1 progenitor.

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