+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis

, : Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis. Plant Journal 8(4): 521-529

A tonoplast protein of 31 kDa apparent molecular mass (TpP 31) was isolated from two-dimensional gels. Amino acid sequences were determined from LysC endoproteinase-peptide fragments. Using degenerate oligonucleotides, a corresponding cDNA clone of 1034 bp was isolated from a barley leaf cDNA library. It encodes for subunit E of the vacuolar H(+)-ATPase, the first one identified in plants so far. The open reading frame extends over 681 bp, encoding a gene product of 227 amino acids and a calculated molecular weight of 26,228 g mol-1. Northern and Western blot analysis indicates constitutive expression of subunit E in all plant organs with only small effects of salt stress. Localization of TpP 31 at the tonoplast was confirmed in fractions of purified vacuolar membrane obtained by free-flow electrophoresis. Immunoprecipitation of newly synthesized 35S-labelled membrane proteins with anti-TpP 31 gave two additional bands with apparent molecular masses of about 53 and 62 kDa. Gel filtration after mild solubilization showed co-purification of TpP 31 with the 55 kDa subunit of the H(+)-ATPase. Both results provide evidence beyond the sequence homology that TpP 31 is a structural component of the vacuolar H(+)-ATPase.


PMID: 7496398

DOI: 10.1046/j.1365-313x.1995.8040521.x

Other references

Harison, R.; Claude, C.; Francois, L., 1998: Madagascar - over 10 years of concentrated institutionalisation with irrigators. The formation of Water Use Associations (WUAs) has been encouraged in Madagascar's irrigation systems (micro-schemes, small schemes and large schemes), to help manage the maintenance of the different system infrastructures. The paper examines...

Hfaiedh Mohamed; Trabelsi Mamadi, 1990: The seismic zones of Tunisia. Pages 403-415 1990

Sparks, L.; Mittapalli, K., 2004: To know or not to know: the case of communication by and with older adult Russians diagnosed with cancer. This paper describes the ways in which group identifications and stereotypes can inform our understanding of cancer treatment and survivorship, as well as the more general social processes surrounding the communicative experiences of older adult R...

María Martín-Moreno, Jé.; Juan Toharia, Jé.; Gutiérrez Fuentes, Jé.Antonio., 2009: Evaluation and prioritisation of the scientific research in Spain. Researchers' point of view. The assessment and prioritisation of research activity are essential components of any Science, Technology and Industry System. Data on researchers' perspectives in this respect are scarce. The objective of this paper was to describe Spanish...

Campbell, J., 1971: Working relationships between providers and consumers in a neighborhood health center. American Journal of Public Health 61(1): 97-103

Weberg, R.; Aubert, E.; Dahlberg, O.; Dybdahl, J.; Ellekjaer, E.; Farup, P.G.; Hovdenak, N.; Lange, O.; Melsom, M.; Stallemo, A., 1988: Low-dose antacids or cimetidine for duodenal ulcer?. In a double-blind, randomized, multicenter trial 150 consecutive outpatients with endoscopically verified duodenal ulcer were treated with either a low-dose antacid regimen (1 tablet q.i.d.; acid-neutralizing capacity, 120 mmol/day), or cimetidine...

Hong, Won Shic, 1992: Plagiochila in western North America. An enumeration of and key to the nine taxa of Western North American Plagiochila are presented. A discussion of distinguishing characteristics and distribution maps of each taxon are provided from specimens examined. Plagiochila satoi var. magnum...

Oganesyan N.M.; Dolabchyan Z.L.; Varosyan M.A.; Tatinyan N.G.; Piruzyan S.A.; Zakharyan A.B.; Sarukhanyan A.G.; Safaryan V.N., 1982: Features of the development of myo cardial hypertrophy during adaptation of the body to high altitude hypoxia. Adaptation of animals [rabbits] to the high-altitude conditions induces myocardium hypertrophy. The degree of this process directly depends on the degree of hypoxia. Data from ECG are correlated with the morphological indices.

Ageev, V.N.; Ignatova, G.V., 1981: Standards for feeding and maintenance of replacement pullets. Replacement pullets were reared on a plane of feeding as recommended by the All-Union Institute of Poultry. It was possible to rear pullets from 45 until 150 days old on diets containing crude protein 14% and metabolizable energy (ME) 1047 kJ/[100...

Evers, K.A., 2012: Report and highlights of the 144th Annual Session of the MSMA House of Delegates and Medical Affairs Forum 2012. Journal of the Mississippi State Medical Association 53(7): 227-232