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Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers


, : Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers. Theoretical and applied genetics 95(5-6): 757-763

The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n = 4x = 28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) using RAPD markers. The first step was to identify amplification of species-specific DNA markers in the parental grass species and durum wheat cultivars. Initially, the genomic DNA of five grass species (Thinopyrum junceiforme, Th. bessarabicum, Lophopyrum elongatum, Leymus karataviensis and Elytrigia pycnantha) and selected durum cultivars ('Langdon', 'Durox', 'Lloyd', 'Monroe', and 'Medora') was screened with 40 oligonucleotide primers (nano-mers). Three oligonucleotides that amplified DNA fragments specific to a grass species or to a durum cultivar were identified. Primer PR21 amplified DNA fragments specific to each of the five durum cultivars, and primers PR22 and PR23 amplified fragments specific to each of the grass species. Intergeneric hybrids between the durum cultivars 'Langdon', 'Lloyd' and 'Durox' and Th. junceiforme, and their backcross (BC) progeny were screened with all 40 primers. Six primers amplified parent-specific DNA fragments in the F1 hybrids and their BC1 progeny. Three primers, PR22, PR23 and PR41, that amplified Th. junceiforme DNA fragments in both F1 and BC1 were further analyzed. The presence of an amplified 1.7-kb Th. junceiforme DNA fragment in the F1 hybrids and BC1 progeny was confirmed using Southern analysis by hybridization with both Th. junceiforme genomic DNA and Th. junceiforme DNA amplified with primer PR41. With the exception of line BC1F2 no. 5. five selfed progeny of BC1 and a BC2 of line 3 (BC1F2 no. 3 x'Lloyd') from a cross of 'Lloyd' x Th. junceiforme showed the presence of the 1.7-kb DNA fragment. All selfed BC1 and BC2 lines retained the 600-bp fragment that was confirmed after hybridization with Th. junceiforme DNA amplified with primer PR22. Other experiments using RFLP markers also showed the presence of up to seven Th. junceiforme DNA fragments in the F1 hybrids and their BC progeny after hybridization with Th. junceiforme DNA amplified with primer PR41. These studies show the usefulness of molecular markers in detecting alien chromatin/DNA fragments in intergeneric hybrids with durum wheat.

US$19.90

DOI: 10.1007/s001220050622


Other references

Balakrishnan, G.; Brownlie, J.; Webber, R.; Gibson, B., 1991: Enhanced thrombin generation in patients receiving intensive care. Thrombin-antithrombin III complex (TAT) concentration was measured in 27 control and 155 intensive care patients to (a) establish normal reference ranges, (b) measure thrombin generation in critically ill patients, and (c) determine the characteri...

Chen, C-Wei.; Yang, H-Chou., 2017: OPATs: Omnibus P-value association tests. Combining statistical significances (P-values) from a set of single-locus association tests in genome-wide association studies is a proof-of-principle method for identifying disease-associated genomic segments, functional genes and biological path...

Dux, A.; Mühlbock, O., 1964: Studies on the influence of genetic factors in the development of cancer of the lacteal glands in mice. Jaarboek van Kankeronderzoek en Kankerbestrijding in Nederland. Yearbook for Cancer Research and Fight Against Cancer in the Netherlands 14: 253-257

Railliet, A.; Henry, A., 1912: Les oesophagostomiens parasites de l'homme. Archives de Parasitologie Paris, 14: 562-583

Hirschowitz, B.I.; Mohnen, J.; Shaw, S., 1996: Long-term treatment with lansoprazole for patients with Zollinger-Ellison syndrome. Background: Normalization of gastric secretion and cure of associated upper gastrointestinal lesions by resection of gastrinoma is possible in apprxeq 20% of patients with Zollinger-Ellison syndrome, leaving ALPHA-PP 80% dependent on medical treat...

Sörman, A.; Zhang, L.; Ding, Z.; Heyman, B., 2014: How antibodies use complement to regulate antibody responses. Antibodies, forming immune complexes with their specific antigen, can cause complete suppression or several 100-fold enhancement of the antibody response. Immune complexes containing IgG and IgM may activate complement and in such situations also...

Sorokina, E.I., 1966: The cardiac pain syndrome in patients with coronary atherosclerosis complicated by inflammation of the sympathetic truncus and its treatment. Sovetskaia Meditsina 29(1): 85-90

Ramsay, A.; Steingart, K.R.; Pai, M., 2011: Assessing the impact of new diagnostics on tuberculosis control. International Journal of Tuberculosis and Lung Disease 14(12): 1506-1507

Cais, M.; Shimoni, M., 1981: A feasible solvent separation system for immunoassays. Feasibility is demonstrated for a new solvent extraction method to separate free and bound fractions in [vertebrate] hapten immunoassays, without the need to centrifuge. A novel mixer-separator device used in conjunction with solvent extraction ma...

Deshpande, A.A.; Dalvi, A.N., 2012: Laparoscopic excision of a giant mesothelial omental cyst. A 42-year-old patient presented with right-sided abdominal discomfort. Investigations revealed a 19 × 21 centimetres large cystic lesion occupying nearly the entire right side of the abdomen. It was situated between ascending colon and right kidn...