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Analysis of the Spm-encoded TnpA and TnpD proteins using single- and double-hybrid protein fusions in a plant transient assay


, : Analysis of the Spm-encoded TnpA and TnpD proteins using single- and double-hybrid protein fusions in a plant transient assay. Maize Genetics Cooperation Newsletter ( 69): 15-17

Tobacco suspension cells were transiently transfected with plasmids containing different putative transcription activation domains of the maize protein TnpA and the GAL4 DNA-binding domain from yeast. The results showed that TnpA lacks a conventional transcription activation domain. However, a new single-hybrid system consisting of the Spm promoter and the TnpA DNA-binding domain could be used to measure transcription activation with greater sensitivity than the GAL4 system.

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