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Development of a PCR assay to detect Fusarium poae in wheat


, : Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology (Oxford) 45(2): 383-391

Random amplified polymorphic DNA assays were carried out on a range of isolates of F. poae to identify markers common to all isolates. Two fragments were isolated, cloned and used to probe Southern blots of DNA from F. poae and isolates from a range of wheat seed and stem base pathogens. One fragment, which hybridized preferentially to DNA of F. poae was partially sequenced and two pairs of primers (Fp8 F/R and Fp82 F/R) were generated for use in the polymerase chain reaction (PCR). Amplification of target DNA occurred following PC R of all isolates of F. poae but not from any of a range of other fungal species associated with diseases of cereal ears and seed. The primer pair Fp82 F/R was used to detect F. poae in extracts from wheat seed samples contaminated with Fusarium species. This system offers the potential to determine the presence of F. poae in wheat and avoid problems commonly associated with conventional diagnosis of the disease and isolation of the pathogen.

US$19.90

DOI: 10.1046/j.1365-3059.1996.d01-133.x


Other references

Chesney, C.M.; Harper, E.; Colman, R.W., 1974: Human platelet collagenase. The presence of proteolytic enzymes such as cathepsin and elastase in platelets and the important role of collagen in platelet aggregation suggested that collagenase might be present in platelets. Epinephrine, ADP, and collagen liberate collagenas...

Iwasaki H.; Igarashi M.; Yamakage M.; Omote K.; Namiki A., 1992: Influence of cardiac output on the onset of neuromuscular blockade after vecuronium. Anesthesiology (Hagerstown) 77(3A): A945

Lu, Z.; Han, J.; Okoromoba, O.E.; Shimizu, N.; Amii, H.; Tormena, Cáudio.F.; Hammond, G.B.; Xu, B., 2017: Predicting Counterion Effects Using a Gold Affinity Index and a Hydrogen Bonding Basicity Index. We have developed a gold affinity index and hydrogen bonding basicity index for counterions and have used these indexes to forecast their reactivity in cationic gold catalysis.

Rabyk, M.; Ostash, B.; Rebets, Y.; Walker, S.; Fedorenko, V., 2012: Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production. The <i>wblA</i><sub><i>gh</i></sub> gene, encoding a homologue of the WhiB-family of proteins, was identified in the sequenced genome of moenomycin producer <i>Streptomyces ghanaensis</i>. Deletion o...

Ellenby, C., 1942: Trace-elements and potato sickness. In soil badly infested with the eelworm Heterodera schachtii potato plants treated with B, Zn, (NH4)SO4, CaSO2, Mn, and Ni grew taller than controls.

Smidt, G.L.; Mommens, M.A., 1980: System of reporting and comparing influence of ambulatory aids on gait. The purposes of this study were to 1) present a standardized approach for describing gait when assistive devices are used, 2) report reference data for unassisted and assisted gait patterns for normal adults, and 3) discuss clinical implications f...

Pilato, G., 1971: Research on the fauna and zoo geography of sicily part 52 tardigrada from fresh water in sicily italy preliminary note. Seven species of freshwater tardigrades found in Sicily [Italy] are described: Isohypsibius deconincki sp. nov., I. matici sp. nov., Macrobiotus richtersi, M. dispar, Doryphoribius zappalai, Isohypsibius angusti and I. tetradactyloides.

Mastrantonio, P.; Pantosti, A.; Cerquetti, M.; Fiorentini, C.; Donelli, G., 1996: Clostridium difficile: an update on virulence mechanisms. Increasing competition in the livestock industry has forced producers to cut costs by adopting new technologies aimed at increasing production efficiency. One particularly promising technology is feeding enzymes as supplements for animal diets. Su...

Solomons, E., 1963: Puerperal thrombophlebitis: prevention and treatment. Postgraduate Medicine 34: 105-111

Nowierski R.M.; Gutierrez A.P., 1986: Numerical and binomial sampling plans for the walnut aphid chromaphis juglandicola homoptera aphididae. Taylor's Power Law was performed on mean and variance estimates of eggs and viviparae of walnut aphids, Chromaphis juglandicola (Kaltenbach). Highly significant correlation coefficients suggested that Taylor's Power Law fit the data well...