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Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphisms


, : Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphisms. Molecular and general genetics: MGG, 253(6): 703-710

A modification of the amplified fragment length polymorphism technique was developed for the determination of DNA methylation in dimorphic fungi representative of three of the major fungal taxa: Mucor rouxii, a zygomycete; Yarrowia lipolytica, an ascomycete; and Ustilago maydis, a basidiomycete. DNA obtained from the yeast or mycelial stages of the fungi was digested with a mixture of EcoRI, and one of the isoschizomers MspI and HpaII, whose ability to cleave at the sequence CpCpGpG is affected by the methylation state. The resulting fragments were ligated to primers and subjected to a double round of amplification by the polymerase chain reaction, radiolabeled in the second round, and separated by polyacrylamide gel electrophoresis. Comparison of patterns revealed differences indicative of fragments whose methylation state did or did not change during the dimorphic transition. These results indicate the usefulness of the method for the study of DNA methylation, demonstrate the universality of DNA methylation in fungi, and confirm that differential DNA methylation occurs during fungal morphogenesis.

US$19.90

PMID: 9079881

DOI: 10.1007/s004380050374


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Zhang, X.R.; Baeyens, W.R.G.; Van Der Weken, G.; Calokerinos, A.C.; Imai, K., 1995: Chemiluminescence determination of some local anaesthetics. The analytical possibilities of the Chemiluminescence observed from some local anaesthetics on acidic permanganate treatment are investigated. Five drugs belonging to the p-aminobenzoate series gave significant emission and the reagent concentrati...

Abou-Arab, Aak, 2001: Release of lead from glaze-ceramicware into foods cooked by open flame and microwave. Samples of ceramicware (24 bowls), purchased from a single manufacturer lot and 36 bowls were collected randomly from a local market in Great Cairo Governorates, Egypt (six locations and six samples from each) and were subjected to different conce...

Wamalwa, D.C.; Farquhar, C.; Obimbo, E.M.; Selig, S.; Mbori-Ngacha, D.A.; Richardson, B.A.; Overbaugh, J.; Egondi, T.; Inwani, I.; John-Stewart, G., 2009: Medication diaries do not improve outcomes with highly active antiretroviral therapy in Kenyan children: a randomized clinical trial. As highly active antiretroviral therapy (HAART) becomes increasingly available to African children, it is important to evaluate simple and feasible methods of improving adherence in order to maximize benefits of therapy. HIV-1-infected children in...

Kaulsay, K.K.; Mertani, H.C.; Törnell, J.; Morel, G.; Lee, K.O.; Lobie, P.E., 1999: Autocrine stimulation of human mammary carcinoma cell proliferation by human growth hormone. Here we have investigated the role of autocrine production of human growth hormone (hGH) in the proliferation of mammary carcinoma cells (MCF-7) in vitro. MCF-7 cells were stably transfected with an expression plasmid encoding the hGH gene, and th...

Downar, J., 2015: Care, compassion, respect. Cmaj 186(17): 1336-1336

O'Dwyer, E.M.; Jones, D.C., 2005: Dental anomalies in Axenfeld-Rieger syndrome. The authors describe the case of a 10-year-old girl presenting with Axenfeld-Rieger syndrome (ARS), a rare autosomal dominant condition. The patient showed severe hypodontia, microdontia and short roots. Early diagnosis of the syndrome from its de...

Zhivkova J.; Kolushev N., 1986: Content of acid soluble nucleotides in placenta of sows on 55th day of gestation. Acid-soluble nucleotides were studied applying two-dimensional paper chromatography. On the 55th day of gestation in placenta of sows were found in 18 free nucleotides. In the examined tissue adenine and guanosine nucleotides followed the same ord...

Evdokimov, V.I.; Zemlyanskii, O.A.; Belyaev, N.B.; Averin, V.A.; Immamedova, N.S.; Makhotin, G.I.; Magula, T.V.; Chemerova, L.I.; Moroz, A.A.; Fetter, N.D.; Firsova, E.I.; Duritsina, T.M.; Romanenko, N.A.; Plyushcheva, G.L.; Novosil'tsev, G.I.; Legon'kov, Y.A.; Rasnitsin, S.P.; Suleimanov, G.D., 1998: The populations migration and the epidemic situation as to parasitoses the Belgorod region. Meditsinskaya Parazitologiya i Parazitarnye Bolezni 0(3): 44-45

Jeffrey K.Rosenfeld (2),, 1979: Interstitial water and sediment chemistry of two cores from Florida Bay. SEPM Journal of Sedimentary Research Vol. 49: 889-917

Broadhurst, M.K.; Larsen, R.B.; Kennelly, S.J.; Mcshane, P.E., 1999: Use and success of composite square-mesh codends in reducing bycatch and in improving size-selectivity of prawns in Gulf St Vincent, South Australia. In response to recent voluntary adoption of square-mesh codends by commercial prawn trawlers in Gulf St. Vincent, Australia, two new designs of composite square-mesh codends were investigated for their utility in 1) reducing the bycatch of fish an...