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Genomic analysis using pulsed-field gel electrophoresis of Escherichia coli 0157:H7 isolated from dairy calves during the United States national dairy heifer evaluation project (1991-1992)

, : Genomic analysis using pulsed-field gel electrophoresis of Escherichia coli 0157:H7 isolated from dairy calves during the United States national dairy heifer evaluation project (1991-1992). Veterinary Microbiology 48(3/4): 223-230

The genomic fingerprints of 26 Escherichia coli 0157:H7 isolates from calves on 20 farms in 16 states were determined by pulsed-field gel electrophoresis (PFGE). Digestion of genomic DNAs with the restriction enzymes SfiI and XbaI yielded 14 and 18 restriction endonuclease digestion profiles (REDP), respectively. 17 farms (85%) had E. coli 0157:H7 with a unique REDP, and when >1 calf tested positive on a farm, the isolates displayed identical REDP.


DOI: 10.1016/0378-1135(95)00135-2

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