+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of a Mycoplasma hyopneumoniae adhesin

, : Identification and characterization of a Mycoplasma hyopneumoniae adhesin. Infection and Immunity 63(3): 1013-1019

An adhesin of Mycoplasma hyopneumoniae was identified and characterized in this study. A monoclonal antibody (MAb), F2G5, and its F(ab')2 fragments inhibited the adherence of M. hyopneumoniae to porcine tracheal cilia, the natural targets to which the mycoplasma binds during infection. MAb F2G5 detected multiple bands, but predominantly recognized a 97-kDa (P97) protein of M. hyopneumoniae on immunoblots. Affinity chromatography, conducted with immobilized MAb F2G5, mainly purified P97. The purified proteins were able to bind to cilia and blocked the adherence of intact M. hyopneumoniae cells to cilia. Immunolabeling of mycoplasmas with MAb F2G5 under electron microscopy demonstrated that the proteins recognized by MAb F2G5 were located at the surface of the mycoplasma, predominantly on a surface fuzzy layer. These results indicate that P97 functions as an adhesin of M. hyopneumoniae. The N-terminal amino acid sequence of P97 did not have significant homology with any known bacterial or mycoplasmal adhesins, suggesting that P97 is a novel protein. The predominant proteins detected by MAb F2G5 in different strains varied in size, indicating that the antigen bearing the epitope for MAb F2G5 undergo intraspecies size variation. Antigenic variation of adhesins may be a pathogenic mechanism utilized by M. hyopneumoniae to evade the porcine immune system.


PMID: 7868222

Other references

Rucker, M.P., 2014: An 18. century method of pain relief in obstetrics. Journal of the History of Medicine and Allied Sciences 5(1): 101-105

Kawasaki, T., 1991: Kawasaki color layer technique. Die Quintessenz der Zahntechnik 17(9): 1089-1102

Thiéblot, L.; Bastide, P.; Blaise, S.; Boyer, J.; Dastugue, G., 1966: On the enzyme activities of the pineal gland. Critical and experimental study. Annales D'endocrinologie 27(1): 1-20

Hwang, S.; Kim, D.Young.; Kim, M.; Chon, Y.Eun.; Lee, H.Jung.; Park, Y-Nyun.; Park, J.Yong.; Ahn, S.Hoon.; Han, K-Hyub.; Chon, C.Yoon., 2010: Deficiencies in proteins C and S in a patient with idiopathic portal hypertension accompanied by portal vein thrombosis. Portal vein thrombosis (PVT) is an uncommon cause of presinusoidal portal hypertension. Among various hepatoportal disorders, noncirrhotic portal hypertension conditions such as idiopathic portal hypertension (IPH) are considered to have a close r...

Toledo, R.; Steinberg, M.P.; Nelson, A.I., 1969: Heat of respiration of fresh produce as affected by controlled atmosphere. A calorimeter was devised to measure the amount of heat evolved by respiring products during continuous flushing in air and controlled atmosphere. The heats of respiration in air determined with this calorimeter were in good agreement with values...

Reddy, S.; Dutta, S.; Narang, A., 2008: Evaluation of lactate dehydrogenase, creatine kinase and hepatic enzymes for the retrospective diagnosis of perinatal asphyxia among sick neonates. It is difficult to make a retrospective diagnosis of perinatal asphyxia in symptomatic neonates delivered non-institutionally. We studied serum creatine kinase muscle-brain fraction (CK-MB), lactate dehydrogenase (LDH), glutamic oxaloacetic transa...

Hartmann, J.T.; Bokemeyer, C., 1999: Chemotherapy for renal cell carcinoma. Renal cell carcinoma is a chemotherapy-resistant tumor, which, with commonly used cytotoxic agents, exhibit a only marginal response rate when modern objective response criteria are applied. No clear survival benefit for patients treated with chem...

Bibby, M.C.; Sleigh,; Loadman, P.M.; Double, J.A., 1993: Potentiation of EO9 anti-tumour activity by hydralazine. EO9[3-hydroxy-5-aziridinyl-1-methyl-2(1H-indole-4,7-dione)prop-bet a-en-alpha-ol] has been selected for phase I evaluation in Europe. Activity has been seen previously in a highly refractory, necrotic mouse adenocarcinoma (MAC 16) but EO9 is shown...

J.L.; Q.W.n; R.J.A.bott; G.R.o, 2013: Geographical distribution of cytotypes in the Chrysanthemum indicum complex as evidenced by ploidy level and genome-size variation. A detailed knowledge of the geographical distribution of cytotypes within and between species comprising a polyploid complex is critical to our understanding of the history and evolution of such complexes. In this study, we examined the geographic...

Pritchard, J.N., 2016: Rethinking the paradigm for the development of inhaled drugs. Nebulized treatment is an important delivery option for the young, elderly, and those with severe chronic respiratory disease, but there is a lack of new nebulized drug products being produced for these patients, leading to the potential for under...