geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In vitro expression and inhibition of procoagulant activity produced by bovine alveolar macrophages and peripheral blood cells


, : In vitro expression and inhibition of procoagulant activity produced by bovine alveolar macrophages and peripheral blood cells. Veterinary Research Communications 20(6): 519-531

Local and systemic activation of coagulation is frequently associated with bacterial sepsis. The coagulopathy is due, at least in part, to expression of tissue factor (TF) by monocytes and macrophages. The purpose of this study was to evaluate the expression of procoagulant activity by bovine alveolar macrophages, leukocytes and platelets, and to determine the relative potency of three chemical inhibitors of TF expression (pentoxifylline, retinoic acid, and cyclosporin A). Bovine alveolar macrophages were stimulated with lipopolysaccharide (LPS) derived from Pasteurella haemolytica or recombinant bovine tumour nervous factor (TNF) and dose- and time-dependent effects on TF expression were studied. LPS and TNF induced TF expression in alveolar macrophages and LPS treatment of whole blood induced TF expression in mononuclear cells. Neutrophils and platelets also expressed procoagulant activity, but this activity was not inhibited by anti-bovine TF monoclonal antibody. Pentoxifylline (40 mu-mol/L), retinoic acid (0.01 mmol/L) and cyclosporin A (0.08 mu-mol/L) inhibited TF expression when added concurrently with LPS or TNF, but not when added 4 h after stimulation. TF mRNA was not detected in unstimulated alveolar macrophages by Northern blot analysis. In contrast, exposure to LPS or TNF for 6 h induced marked expression of TF mRNA, which was inhibited by treatment with pentoxifylline, retinoic acid and cyclosporin A. Expression of TNF by alveolar macrophages stimulated with LPS was also inhibited by these compounds. Our results indicate that procoagulant activity expressed by alveolar macrophages and monocytes is associated with expression of TF, whereas procoagulant activity expressed by neutrophils and platelets is not. The concentrations of pentoxifylline and retinoic acid necessary for inhibition of TF expression in vitro, may not be achievable in vivo owing to their toxic effects. However, the in vitro concentration of cyclosporin A that inhibited TF expression did not exceed the plasma concentration observed in humans, and therefore may be useful for inhibition of TF expression in vivo.

US$19.90

PMID: 8950833

DOI: 10.1007/bf00396295


Other references

Ishii, H.; Akiba, T.; Marushima, H.; Kanetsuna, Y.; Morikawa, T., 2012: A case of bilateral multiple pulmonary chondroma: necessity of follow-up for Carney's triad. We report a case of pulmonary bilateral multiple chondromas that were possibly an initial clinical presentation of Carney s triad. A 56-year-old Japanese non-smoking asymptomatic woman was admitted to the hospital for further examination of small,...

Moussally, K.; Oraichi, D.; Bérard, A., 2009: Herbal products use during pregnancy: prevalence and predictors. (1) Measure the prevalence of herbal product (HP) use, alone, and concomitantly with prescribed medications during pregnancy, (2) identify the most frequently consumed HP during gestation and (3) determine predictors of HP use at the beginning of...

McFadden, S.H., 2000: Religion, personality, and aging: a life span perspective. A life span model of development provides the framework for examining the relation between religion and personality in aging persons. This article emphasizes the interplay of constancy and change in adult religious life. Important contextual facto...

Breivik, H.; Rennemo, F., 1982: Clinical evaluation of combined treatment with methadone and psychotropic drugs in cancer patients. Acta Anaesthesiologica Scandinavica. Supplementum 74: 135-140

Shaw, Mark R., 2006: Notes on British Pimplinae and Poemeniinae (Hymenoptera: Ichneumonidae), with additions to the British list. British (sensu British Isles) species of two ichneumonid subfamilies, Pimplinae and Poemeniinae, treated as "Pimplinae" in a Royal Entomological Society Handbook for the Identification of British Insects (Fitton. Shaw & Gauld, 1988), are...

Fujii, Kikue, 1963: ABO and Lea blood group antigens in cell granules of human liver. Cells of the human liver were separated by means of differential centrifugation into 4 fractions; a nucleus fraction, a large granule fraction, a microsome fraction, and a supernate fraction. Lipids of large granule and microsome fractions were ca...

Ekramoddoullah, A.K.; Kisil, F.T.; Sehon, A.H., 1986: Partial characterization of an antigenic site of high molecular weight basic antigen, a ryegrass pollen allergen, using a monoclonal antibody. We have previously reported on the production of murine monoclonal antibodies (Mabs) to the retentate fraction of the aqueous extract of Kentucky bluegrass pollen (KBG-R) [Kisil et al. (1980) Fedn Proc. 39, 3479]. In the present study, the ability...

Grant, E.; Lilly, S.T.; Herath, S.; Sheldon, I.M., 2007: Escherichia coli lipopolysaccharide modulates bovine luteal cell function. Prolonged luteal phases of oestrous cycle associated with postpartum metritis and endometritis are an important cause of subfertility in cattle. These uterine diseases are commonly associated with the presence of Escherichia coli and high concentr...

Tandon, Rakesh K., 1999: Herbal medicine in the treatment of viral hepatitis. Journal of Gastroenterology & Hepatology 14(Suppl A): A291-A292, Dec

Sokolenko, O.K.; Furmanenko, N.F.; Zaritskiĭ, G.V.; Konovalenko, V.V., 1978: Giant cysts of the ovaries. Klinicheskaia Khirurgiia: 76-77