geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In vitro method for quantification of the fermentation of starch by human faecal bacteria


, : In vitro method for quantification of the fermentation of starch by human faecal bacteria. Journal of the Science of Food & Agriculture 71(2): 209-217

The major actions of undigested carbohydrates, such as non-starch polysaccharides and resistant starch, on the physiology of man are related to colonic fermentation. It is very difficult to study fermentation in vivo. In vitro models are often used but these models differ in almost every aspect even when only the simplest batch cultures of human faeces are considered. Factors such as composition of the medium, buffering capacity, inoculum concentration, and fermentation time vary considerably making direct comparison of the results from different studies impossible. The authors have devised a standard in vitro fermentation method for resistant starch and tested it in eight laboratories with a total of 40 individual faecal inocula. This simple method was well received and taking biological variation into account yielded comparable results for 24 h short chain fatty acid production and residual starch in all but one laboratory.

US$19.90

DOI: 10.1002/(sici)1097-0010(199606)71:2<209::aid-jsfa571>3.0.co;2-4


Other references

Busch W.; Stolze R., 1969: Experiments in preservation of boar semen. Fortpflanzung Besamung und Aufzucht der Haustiere 5(4): 212-224

Chumak, V.; Kotsar'-, V., 1979: Comparative effectiveness of broadcast and local application of fertilizers in maize cultivation. Biulleten' Vsesoiuznogo nauchno issledovatel'skogo instituta kukuruzy: ) 26-28

Brewer, D.; Mccoll, S.; Wennerberg, A., 1996: Blood administration monitoring A paradigm shift. Transfusion (Bethesda) 36(9 SUPPL ): 79S

Geczy, B.; Meister, C., 1998: Domerian ammonites from the Bakony Mountains Hungary Les ammonites du Domerien de la montagne du Bakony Hongrie. The study of Domerian ammonites from 6 localities of the Bakony Mountains, allows to describe 11 faunal horizons based on the stratigraphicai succession of more than 65 taxa. These 11 biostratigraphical units can be correlated without problems wit...

Anonymous, 1995: Poisoning the greenhouse. Science 268(5210): 503-505

Kamath, P.S., 2005: Esophageal variceal bleeding: primary prophylaxis. Clinical Gastroenterology and Hepatology 3(1): 90-93

Vasil Eva L.K., 1969: Myo cardium functional capacity during chronic nonspecific pulmonary diseases. Vrachebnoe Delo: 38

Miura, H.; Arai, S.; Funano, F., 1973: Restoration of stainability of histologic tissue specimen following fixation. Rinsho Byori. Japanese Journal of Clinical Pathology 21(4): 379-382

Umrath, Karl, 1933: Plasmolytische Versuche an Eierstockeiern von Bufo vulgaris. By plasmoly-sis of unripe eggs of B. vulgaris in the follicle with CaCl2, NaCl, or KCl in 10 times isotonic conc., the egg remains in the follicle with the surface markedly folded and with the protoplasm punctated. The result differs but little wi...

Ammerman, Cb, 1978: Indicators of mineral deficiencies: what about hair?. Feed management including feed age Eastern edition: 29 (11) 17-18