+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.)

, : Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). Plant Cell Reports 16(8): 565-571

The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influcnced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly in fluenced the shoot regeneration response. The optimum BA concentration for shoot/shoot bud regeneration was genotype dependent. Two types of BA-induced response were observed: (1) at less than 7.5 micromolar BA, direct shoot differentiation (2 to 4-cm-long shoots) was observed within 30 days: (2) at higher BA concentrations (75-100 micromolar), shoot/shoot bud differentiation was achieved in 45-90 days. A high BA concentration inhibited subsequent rooting of shoots. Roots, however, could be easily induced on shoots derived from < 12.5 micromolar BA. Following transfer to soil, 80% of the regenerants developed into morphologically normal and fertile plants.


DOI: 10.1007/BF01142325

Other references

Offner, F.; Moisand, B., 1966: A coincidence technique for fetal electrocardiography. American Journal of Obstetrics and Gynecology 95(5): 676-680

Páv, J., 1973: Possibilities of prevention of diabetes mellitus by nutrition. Ceskoslovenska Gynekologie 27(5): 337-341

Ratner, G.L.; Berezin, I.M., 1972: Remote results of selective vagotomy with draining operations on the stomach in peptic ulcer. Khirurgiia 48(1): 123-127

Nakamura, S.; Racker, E., 1984: Inhibitory effect of duramycin on partial reactions catalyzed by (Na+,K+)-adenosinetriphosphatase from dog kidney. The polypeptide antibiotic duramycin inhibited the (Na+,K+)-adenosinetriphosphatase purified from dog kidney. An analysis of its mode of action revealed that the formation of phosphoenzyme from Pi but not from ATP was inhibited. The rate of dephos...

Lucas, H., 1881: Atax histrionicus, Herm.: note by H. Lucas, Bull. Soc. Ent. Fr. 5 x. p. liv. A. crassipes, Mull.:. Atax histrionicus, Herm.: note by H. Lucas, Bull. Soc. Ent. Fr. (5) x. p. liv. A. crassipes, Mull.: F. Konike notes glands found in this species, similar to those found among the Hydrachnidae, but excessively developed; Zool. anz. iv. pp. 356 & 35...

Tedford, C.E.; Pawlowski, G.P.; Khan, M.A.; Phillips, J.G.; Ali, S.M.; Fadnis, L.; Gregory, R.; Handley, M.K.; Hirth, W.W.; Edgar, D.M.; Siedel, W.F.; Yates, S.L., 1997: Pharmacological characterization of GT-2227, a novel histamine H-3 receptor antagonist. Society for Neuroscience Abstracts 23(1-2): 1784

Kowalska, J.; Zabłocka, A.; Wilusz, T., 2006: Isolation and primary structures of seven serine proteinase inhibitors from Cyclanthera pedata seeds. Seven new trypsin inhibitors, CyPTI I-VII, were purified from ripe seeds of Cyclanthera pedata by affinity chromatography on immobilized chymotrypsin in the presence of 5 M NaCl followed by preparative native PAGE at pH 8.9. The CyPTIs (Cyclanther...

Orr, L.M.; Mathers, F.; Butt, T.C., 1949: Somatic pain produced by fibrolipomatous nodules simulating urinary tract pathology. Southern Surgeon 15(10): 737-748

Dello Russo, N.M., 2010: Not a court of law. Journal of the American Dental Association 140(11): 1344; Author Reply 1347-1344; Author Reply 1347

Jenkins, R.A.; Carey, J.W., 2005: Decision making for HIV prevention planning: organizational considerations and influencing factors. The purpose of this CDC-funded project was to better understand how behavioral data were used in HIV prevention community planning, and use this knowledge to develop and evaluate tools for increasing the use of data in HIV prevention planning. HIV...