geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Overexpression of cellular glutathione peroxidase does not affect expression of plasma glutathione peroxidase or phospholipid hydroperoxide glutathione peroxidase in mice offered diets adequate or deficient in selenium


, : Overexpression of cellular glutathione peroxidase does not affect expression of plasma glutathione peroxidase or phospholipid hydroperoxide glutathione peroxidase in mice offered diets adequate or deficient in selenium. Journal of Nutrition 127(5): 675-680

Selenium-dependent cellular glutathione peroxidase (GPX1) overexpressing [GPX1(+)] mice were derived by microinjecting a 5.3-kb cloned entire mouse GPX1 genomic DNA into fertilized eggs. The objective of this study was to determine the effect of GPX1 overexpression and dietary selenium on the expression of selenoperoxidases and the status of lipid peroxidation of these transgenic animals. An experiment with a 2 x 2 factorial arrangement of treatments with 15 GPX1(+) and 15 control mice (2 mo old) was conducted for 8 wk. Ten mice of each group (half males and females) were fed a Se-deficient, Torula yeast basal diet (0.02 mg Se/kg, no supplemental vitamin E) and five mice (three males and two females) were fed the basal diet supplemented with 0.51 mg Se/kg as Na2SeO3. The GPX1(+) mice had greater GPX1 activities (one- to sixfold, P < 0.0001) than the control mice at both levels of dietary selenium in all tissues except for liver, in which such difference (100%, P < 0.05) was observed only in Se-deficient mice. The GPX1 mRNA level in kidney and in lung of the Se-deficient GPX1(+) mice was 81% and 7.5-fold greater (P < 0.003) than the respective control level. Overexpression of GPX1 did not alter phospholipid hydroperoxide glutathione peroxidase (GPX4) activities and mRNA levels or glutathione S-transferase (GST) activities in most of the tissues, plasma glutathione peroxidase (GPX3) activity or plasma Se concentrations. No differences in lipid peroxidation in kidney, lung or intestine were observed between the Se-deficient GPX1(+) and control mice. In conclusion, the overexpression of the GPX1 gene in these mice was tissue specific and did not affect the expression of GPX3, GPX4 or GST and plasma Se levels; dietary Se appeared to affect the GPX1 overexpression at its mRNA level.

US$29.90

PMID: 9164985


Other references

MacTaggart, J.N.; Pipinos, I.I.; Johanning, J.M.; Lynch, T.G., 2006: Acrylic cement pulmonary embolus masquerading as an embolized central venous catheter fragment. An acrylic cement pulmonary embolus is a rare complication of percutaneous vertebroplasty. Many patients remain completely asymptomatic, but others may present with chest discomfort, dyspnea, and even cardiovascular collapse leading to death. Here...

Healy, M.G.; Ryan, P.C.; Fenton, O.; Peyton, D.P.; Wall, D.P.; Morrison, L., 2016: Bioaccumulation of metals in ryegrass (Lolium perenne L.) following the application of lime stabilised, thermally dried and anaerobically digested sewage sludge. The uptake and accumulation of metals in plants is a potential pathway for the transfer of environmental contaminants in the food chain, and poses potential health and environmental risks. In light of increased population growth and urbanisation,...

Cao, J.; Xiao, L.; He, B.; Zhang, G.; Dong, J.; Wu, Y.; Xie, H.; Wang, G.; Lin, X., 2017: Diagnostic value of combined diffusion-weighted imaging with dynamic contrast enhancement MRI in differentiating malignant from benign bone lesions. To determine the diagnostic value of combined diffusion-weighted imaging (DWI) with dynamic contrast enhancement magnetic resonance imaging (DCE-MRI) in differentiating malignant from benign bone lesions. DWI and DCE-MRI were performed in 36 patie...

Lurhuma Z.; Shafik A.; Diese M.; Wane J.; Tshiamala K.; Bimamisana B., 1989: The effect of mm 1 on patients with aids. Morisset, R A (Ed ) Ve Conference Internationale Sur Le Sida: Le Defi Scientifique Et Social; V International Conference on Aids: The Scientific And Social Challenge; Montreal, Quebec, Canada, June 4-9, 1262p International Development Research Centre: Ottawa, Ontario, Canada Illus Paper 404

Ali, F.; Akhter, W.; Arshad, M., 2015: Magnetic resonance cholangiopancreatography in diagnosis of biliary disorders in children - Sharing our experience. To evaluate the role of Magnetic Resonance Cholangio-pancreatography in the diagnosis of biliary disorders in children. The retrospective study was conducted at Aga Khan University Hospital, Karachi, and comprised data related to the period betwee...

Schmitt, Michael., 1994: Die unendliche Synthese - Erweiterungen der Evolutionstheorie seit 1950. Berichte der Naturforschenden Gesellschaft zu Freiburg im Breisgau. 1992-1993( ); 82-83: 169-192

Yamamoto, S.; Matsumoto, K.; Sakamoto, S.; Tarutani, K.; Minato, K.; Senda, M., 2005: An intra-operative positron probe with background rejection capability for FDG-guided surgery. For radio-guided surgery on tumors using F-18-FDG, detection of annihilation gamma photons emanating from other parts of the body produces background radiation counts and limits its use in clinical situations. To overcome this limitation, we have...

Zhao, Y.; Kato, S.; Zhao, J., 2016: Characteristics of particle coagulation in an underground parking lot. Particles in vehicle exhaust plumes in underground parking lots have adverse health effects due to the enclosed environment in which they are released and the temperature difference between the tailpipe and ambient environment; at the same time, p...

Kvistad, C.E.; Oeygarden, H.; Logallo, N.; Thomassen, L.; Waje-Andreassen, U.; Naess, H., 2015: Is smoking associated with favourable outcome in tPA-treated stroke patients?. Smoking has been associated with improved outcome in thrombolysed patients with myocardial infarction and higher recanalization rates in stroke patients treated with tissue plasminogen activator (tPA). We hypothesized a positive association betwee...

Eriksson, A.W.; Fellman, J.; Nieminen, H.; Forsius, H., 1965: Influence of age on the position and size of the iris frill and the pupil. Acta Ophthalmologica 43(5): 629-641