+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Plant 21D7 protein, a nuclear antigen associated with cell division, is a component of the 26S proteasome

, : Plant 21D7 protein, a nuclear antigen associated with cell division, is a component of the 26S proteasome. Plant Physiology 113(1): 281-291

Previously, we cloned a carrot (Daucus carota L.) cDNA encoding a 45-kD protein, 21D7, located in the nuclei of proliferating cells. The 21D7 protein is similar to the partial sequence of a regulatory subunit of the bovine 26S proteasome, p58 (G. DeMartino, C.R. Moomaw, O.P. Zagnitko, R.J. Proske, M. Chu-Ping, S.l. Afendis, I.C. Swaffield, C.A. Slaughter [1994] J Biol Chem 269: 20878-20884) and to the deduced sequence encoded by the Saccharomyces cerevisiae gene SUN2 (M. Kawamura, K. Kominami, J. Takeuchi, A. Toh-e [1996] Mol Gen Genet 251: [146-152]). In our work, the expression of plant 21 D7 cDNA rescued the yeast sun2 mutant. Fractionation of carrot and spinach (Spinacia oleracea L.) crude extracts showed that the 21D7 protein sedimented with the active 26S proteasomes. The cessation of cell proliferation in carrot suspensions at the stationary phase caused 26S proteasome dissociation and, correspondingly, the 21D7 protein sedimented together with the free regulatory complexes of the 26S proteasomes. Large-scale purification of carrot 26S proteasomes resulted in coisolation of the 21D7 protein. Polyacrylamide gel electrophoresis under nondenaturing conditions showed that the 21D7 protein had the same mobility as the 26S proteasome and that proteasome dissociation changed the mobility of the 21D7 protein accordingly. We conclude that the 21D7 protein is a subunit of the plant 26S proteasome and that it probably belongs to the proteasome regulatory complex.


PMID: 9008397

DOI: 10.1104/pp.113.1.281

Other references

Cavic, B.A.; Thompson, M., 2000: Adsorptions of plasma proteins and their elutabilities from a polysiloxane surface studied by an on-line acoustic wave sensor. Gold electrodes of thickness-shear mode acoustic wave sensors were modified with poly[(mercaptopropyl)methylsiloxane]. The flow-through adsorption of three major plasma proteins (human serum albumin, fibrinogen, and immunoglobulin G) was detected...

Abete, I.; Parra, D.; Crujeiras, A.B.; Goyenechea, E.; Martinez, J.A., 2008: Specific insulin sensitivity and leptin responses to a nutritional treatment of obesity via a combination of energy restriction and fatty fish intake. Background: Nutritional strategies to treat obesity often influence neuroendocrine factors related to body weight control. The present study aimed to investigate whether the inclusion of three fatty fish servings per week within a hypocaloric diet...

Magrath, I.; Barriga, F.; McManaway, M.; Shiramizu, B., 1988: The molecular analysis of chromosomal translocations as a diagnostic, epidemiological and potentially prognostic tool in lymphoid neoplasia. Neoplasms have been shown to be associated with specific non-random chromosomal abnormalities. The present paper summarizes molecular consequences of chromosomal translocations in lymphoid malignancies, especially in Burkitt's lymphoma. Among...

Ray, S.; Jerome, VE.; Thorne, JA., 1984: Copper, zinc, cadmium, and lead in scallops (Placopecten magellanicus) from Chaleur Bay, New Brunswick. International Council for the Exploration of the Sea C.M.: /E:22: 1-15

Fentress, V.; Barrett, R.; Sultzman, C., 1961: Hereditary exostosis complicated by pleural effusion and diaphragmatic rupture-case report. Harper Hospital Bulletin 19: 180-184

Shen, H.; Harvey, B.K.; Chiang, Y-Hsiao.; Pick, C.G.; Wang, Y., 2011: Methamphetamine potentiates behavioral and electrochemical responses after mild traumatic brain injury in mice. We previously demonstrated that high doses of methamphetamine (MA) exacerbate damage induced by severe brain trauma. The purpose of the present study was to examine if MA, at low dosage, affected abnormalities in locomotor activity and dopamine tu...

Landaburu R.H.; Quintana C.M.; D.L.ndaburu C.A.; Castellanos D.E., 1971: Neuro humoral control of blood coagulation part 3 prothrombin synthesis. Acta Physiologica Latinoamericana 21(4): 339-345

Wylie, R.J., 1978: Federal action in regulating ethylene oxide. Journal of the Parenteral Drug Association 32(6): 291-294

Rejeski, W.Jack., 2008: Mindfulness: reconnecting the body and mind in geriatric medicine and gerontology. Derived from Buddhism, mindfulness is a unique approach for understanding human suffering and happiness that has attracted rapidly growing interest among health care professionals. In this article I describe current thinking about the concept of m...

Guarnaschelli, C.; D.P.trizi, S.; Felicetti, G.; Achilli, M.P., 1995: A computerized endurance test: efficacy of pelvic floor rehabilitation treatment in patients with stress incontinence. In the present study the authors wanted to transfer rigorous methods of study, already in use in other sectors (sports, medicine, isokinetic work, etc.) of the validity of interventions made and their effectiveness, into an "emerging" fi...