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Polymerase chain reaction: amplification and sequencing of the VP1 gene of foot-and-mouth disease virus type A


, : Polymerase chain reaction: amplification and sequencing of the VP1 gene of foot-and-mouth disease virus type A. Bulletin of the Veterinary Institute in Puawy 40(2): 91-96

A polymerase chain reaction (PCR) method was used in the detection of foot and mouth disease (FMD) viral RNA in samples taken from a calf during the clinical stage of FMD following experimental infection with a Polish strain of FMD virus type A. Total RNA was extracted using a guanidinium thiocyanate-phenol-chloroform method and was reverse transcribed using AMV-reverse transcriptase. cDNA was used as a template for the amplification by PCR of the 672 bp of the VP1 coding sequence.

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