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Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events

, : Transformation of Penicillium freii and a rapid PCR screening procedure for cotransformation events. Microbiological Research 151(3): 281-284

A strain of Penicillium freii was transformed to hygromycin B resistance using the pAN7-1 plasmid. The transformation frequency was approximately 100 transformants per microgram DNA per 1 x 10(7) protoplasts. Southern blotting revealed different types of integration into the genome of P.freii. When non-selectable plasmid DNA was added together with pAN7-1, this resulted in a cotransformation frequency of more than 60%. The cotransformation frequency was estimated using a facile DNA extraction method together with PCR.


PMID: 8817919

DOI: 10.1016/S0944-5013(96)80025-5

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