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Effect of different plant species from natural grassland on rumen microbial population


, : Effect of different plant species from natural grassland on rumen microbial population. Ecological and biological aspects of fodder crop production Refereed papers from an international research conference held at Nitra, Slovakia, 23 October 1997: 157-161

A laboratory method for the estimation of the in vitro effect of plant soluble fraction compounds on microbial growth is described. The soluble fraction of the dried plant material from each of 27 grasses, legumes and forbs was extracted using anaerobic McDougall's buffer (artificial saliva) and anaerobic fermentation of the plant extract and strained rumen fluid mixture was carried out for 24 h at 39 degrees C with slow agitation. The microbial culture was isolated by centrifugation.

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