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Study of high-molecular weight compounds in sugar using gel-permeation chromatography with an evaporative light scattering detector

, : Study of high-molecular weight compounds in sugar using gel-permeation chromatography with an evaporative light scattering detector. Carbohydrate Polymers 37(3): 257-261, Nov

Raw sugars and other sugar process materials are studied by GPC (Gel Permeation Chromatography) using a Superose 12 column. As eluent was used a solution of 30% acetonitrile with 0.005 M ammonium acetate. As detector was used to spectrophotometric Diode Array Detector (DAD) and an Evaporative Light Scattering Detector, in series. By this arrangement both chromophoric and non chromophoric compounds are detected simultaneously. High sensitivity of both detectors allows a rapid detection of high molecular weight compounds without pre concentration of samples.


DOI: 10.1016/s0144-8617(98)00068-x

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