+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation

, : The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation. Proceedings of the National Academy of Sciences of the United States of America8, 96(20): 11416-11421

The yeast and human SKP1 genes regulate the mitotic cell cycle but are not yet known to be required for meiosis. Nine Arabidopsis SKP1 homologues have been uncovered and are named ASK1 through ASK9. Here, we report the isolation and characterization of a male sterile Arabidopsis mutant and show that the mutant defect was caused by a Ds transposon insertion into the ASK1 gene. In the ask1-1 mutant, abnormal microspores exhibit a range of sizes. Furthermore, during mutant male meiosis, although homologous chromosome pairing appeared normal at metaphase I, chromosome segregation at anaphase I is unequal, and some chromosomes are abnormally extended. Therefore, in ask1-1, at least some homologues remain associated after metaphase I. In addition, immunofluorescence microscopy indicates that the mutant spindle morphology at both metaphase I and early anaphase I is normal; thus, the abnormal chromosome segregation is not likely caused by a spindle defect. Because the yeast Skp1p is required for targeting specific proteins for ubiquitin-mediated proteolysis, we propose that ASK1 controls homologue separation by degrading or otherwise removing a protein that is required directly or indirectly for homologue association before anaphase I.


PMID: 10500191

DOI: 10.2307/49051

Other references

Henik, W.; Domino, G., 1975: Alterations in future time perspective in heroin addicts. Changes in the future time perspective of 31 narcotic addicts who were participating in a methadone maintenance treatment program were studied by a 9-week test-retest design that used a matched control group of waiting list addicts. Significant ch...

Duclos M.; Tetu B.; Raymond P.E., 1991: Granulosa cells tumor a rare entity. Clinical & Investigative Medicine 14(4 SUPPL): A108

Martin, J.H.; Ghez, C., 1988: Red nucleus and motor cortex: parallel motor systems for the initiation and control of skilled movement. This study examines the differential contributions of motor cortex (MCx) and red nucleus (RN) neurons to the initiation of a targeted limb response and to the control of trajectory. These questions were assessed in two ways. First, by comparing th...

Capello Carlo Felice, 1939: Grotte e caverne delle valli delle Dore Baltea e Riparia. The caves of the Dora Baltea and Dora Riparia valleys, Piedmont, Italy, occur in different kinds of rocks and differ in origin and structure. Although most of them are karst phenomena in Triassic sediments, others occur in rocks of various ages.

Bennett, B.; Misra, S.; Brashears, A.D.wty, T., 1995: Effect of bur extractor on trash in seed cotton and fiber quality for different harvest dates. Proceedings (1): 649-652

Bohle, B., 2004: T lymphocytes and food allergy. Food allergy is a hypersensitivity reaction to normally harmless substances and involves humoral immune responses, mediated by immunoglobulin (IgE) synthesized by B lymphocytes, and cellular immune responses mediated by T lymphocytes. An IgE-media...

Androsova, E.H., 1955: Polyzoa of the Order Cheilostomata of the northern part of the Sea of Japan. Inst Acad. Sci. U.S.S.R. Dissertation : 3-13

Joksimović, M., 1968: Significance of spirometric tests in the early detection of obstructive conditions. Diagnostic value of Hutchinson's spirometer. Medicinski Glasnik 22(12): 427-428

Hickam, J.B.; Deiss, W.P.; Frayser, R., 1965: Intramural Use Of Extramural Examinations. JAMA 192: 828-831

Martínez-Manautou, J.; Maqueo, M.; Aznar, R.; Pharriss, B.B.; Zaffaroni, A., 1975: Endometrial morphology in women exposed to uterine systems releasing progesterone. A blind study was done in 402 endometrial biopsies of women who had intrauterine devices releasing different daily amounts of progesterone or a placebo (empty device): 175 were obtained in what presumably was the proliferative phase and 227 in the...