geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A novel cytosolic dual specificity phosphatase, interacting with glucokinase, increases glucose phosphorylation rate


, : A novel cytosolic dual specificity phosphatase, interacting with glucokinase, increases glucose phosphorylation rate. Journal of Biological Chemistry 275(42): 32406-32412

A novel protein was cloned from a rat liver cDNA library by interaction with the liver glucokinase. This protein contained 339 residues and possessed a canonical consensus sequence for a dual specificity phosphatase. The recombinant protein was able to dephosphorylate phosphotyrosyl and phosphoseryl/threonyl substrates. We called this protein glucokinase-associated phosphatase (GKAP). GKAP partially dephosphorylated a recombinant glucokinase previously phosphorylated, in vitro, by protein kinase A. GKAP fused with green fluorescent protein was located in the cytosol, where glucokinase phosphorylates glucose, and not in the nucleus, where the glucokinase is kept inactive by the glucokinase regulatory protein. GKAP accelerated the glucokinase activity in a dose-dependent manner and with a stoichiometry compatible with a physiological mechanism. This strongly suggested that the interaction between GKAP and glucokinase had a functional significance. The cloning of this novel protein with a dual specificity phosphatase activity allows the description of a possible new regulatory step in controlling the glycolysis flux. The nucleotide sequence reported has been deposited with the EBI/GenBank databases with the accession number AF217233.

US$19.90

PMID: 10913113

DOI: 10.1074/jbc.M000841200


Other references

Pei, Y-Fang.; Zhang, L.; Liu, Y.; Li, J.; Shen, H.; Liu, Y-Zhong.; Tian, Q.; He, H.; Wu, S.; Ran, S.; Han, Y.; Hai, R.; Lin, Y.; Zhu, J.; Zhu, X-Zhen.; Papasian, C.J.; Deng, H-Wen., 2014: Meta-analysis of genome-wide association data identifies novel susceptibility loci for obesity. Obesity is a major public health problem with strong genetic determination. Multiple genetic variants have been implicated for obesity by conducting genome-wide association (GWA) studies, primarily focused on body mass index (BMI). Fat body mass (...

Yang, Z-Xin.; Ji, Y-Ru.; Han, Z-Bo.; Wang, Y-Wei.; Meng, L.; Han, Z-Chao., 2014: Long term in-vitro expansion reduces immune modulation function of placental chorionic villi mesenchymal stem cells. The main aim of this study was to investigate the biological activities and immune modulation changes of chorionic villi mesenchymal stem cells (CV-MSC) after long term culture. The morphology of the CV-MSC of passage 3 and passage 9 were observed...

Glennon, R.A.; Titeler, M.; Lyon, R.A.; Slusher, R.M., 1988: N,N-di-n-propylserotonin: binding at serotonin binding sites and a comparison with 8-hydroxy-2-(di-n-propylamino)tetralin. 8-Hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) is a serotonergic agonist with high affinity and selectivity for a particular population of central serotonin (5-HT) binding sites (i.e., 5-HT1A sites). Because the selectivity of 8-OH-DPAT may be...

Pennycook, S.R.; Triggs, C.M., 1992: Bacterial blossom blight of kiwifruit - a 5-year survey. The incidence of bacterial blossom blight (Pseudomonas viridiflava) was surveyed in kiwifruit orchards in the Te Puke district, Bay of Plenty, New Zealand, during 1984-88. In each year, at least 60 orchards were included in the survey with some be...

Thot A.; Rubanyi G.; Kovach A.G.B., 1980: Contractile activity and metabolism in the pregnant rat uterus. Acta Physiologica Academiae Scientiarum Hungaricae 56(1): 92-93

Thomas Gillgren; Marie-Victoire Faye; Mats Stading, 2011: Mechanical and Barrier Properties of Films from Millet Protein Pennisetin. Plastics are one of the most commonly used materials today in an immense range of applications. Since plastics originate from petroleum, which is not a renewable resource, we need to find alternatives to achieve environmentally sustainable goals....

Q.H.iMin; Zhao TingTing; Zhang QuanBin; L.Z.iEn; Zhao ZengQin; Xing RonGe, 2005: Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta). Polysaccharides extracted from Ulva pertusa Kjellm (Chlorophyta) are a group of sulfated heteropolysaccharides, the ulvans. In this study, different molecular weight ulvans were prepared by H2O2 degradation and their antioxidant activities investi...

Bronzino, J.D.; Stern, W.C.; Leahy, J.P.; Morgane, P.J., 1976: Sleep cycles in cats during chronic electrical stimulation of the area postrema and the anterior raphe. Sleep-waking profiles were obtained from 130 7 h stimulation-EEG recording sessions in a series of cats bearing chronically implanted stimulating electrodes in the regions of the area postrema and anterior raphe nuclei. During electrical stimulati...

Burtscher, J.; Sweeney, R.; Bale, R.; Eisner, W.; Twerdy, K., 2003: Neuroendoscopy based on computer assisted adjustment of the endoscope holder in the laboratory. We present our initial clinical experience with a novel technique of frameless stereotactic neuroendoscopy using a neuronavigation system, a specially designed aiming device (endoscope holder/targeting device) combined with a vacuum-mouthpiece bas...

Anonymous, 1992: Congress 1992--the unique person. Sygeplejersken 92(25): 18-22