geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A simplified, sequential, phosphorus fractionation method


, : A simplified, sequential, phosphorus fractionation method. Communications in soil science and plant analysis 31(11-14): 1981-1991

Hedley et al. (1982) developed what has become the most widely used (and modified), phosphorus (P) fractionation technique. It consists of sequential extraction of increasingly less phytoavailable P pools. Extracts are centrifuged at up to 25000 g (RCF) and filtered to 0.45 micrometer to ensure that soil is not lost between extractions. In attempting to transfer this method to laboratories with limited facilities, it was considered that access to high-speed centrifuges, and the cost of frequent filtration may prevent adoption of this P fractionation technique. The modified method presented here was developed to simplify methodology, reduce cost, and therefore increase accessibility of P fractionation technology. It provides quantitative recovery of soil between extractions, using low speed centrifugation without filtration. This is achieved by increasing the ionic strength of dilute extracts, through the addition of NaCl, to flocculate clay particles. Addition of NaCl does not change the amount of P extracted. Flocculation with low speed centrifugation produced extracts comparable with those having undergone filtration (0.025 micrometer). A malachite green colorimetric method was adopted for inorganic P determination, as this simple manual method provides high sensitivity with negligible interference from other anions. This approach can also be used for total P following digestion, alternatively non-discriminatory methods, such as inductively coupled plasma atomic emission spectroscopy, may be employed.

US$29.90


Other references

Masuda, R.; Kaneko, K., 1997: Asparagine degrading activities in some developing soybean. Plant Physiology (Rockville) 114(3 SUPPL ): 79

Victor P.Pasko, Yoav Yair, Cheng-Ling Kuo, 2012: Lightning Related Transient Luminous Events at High Altitude in the Earth’s Atmosphere Phenomenology, Mechanisms and Effects. This paper presents a literature survey on the recent developments related to experimental and modeling studies of transient luminous events (TLEs) in the middle atmosphere termed elves, sprites and jets that are produced in association with thund...

Thanapaisal, C.; Wongkonkitsin, N.; Seow, O.T.r-Sae; Rangsrikajee, D.; Jenwitheesuk, K.; Phugkhem, A.; Bhudisawadi, V., 2005: Outcome of in-patient trauma cases: Accident and emergency unit, Khon Kaen University. Objectives: To study and report the outcome of in-patient trauma cases based on the Trauma and Injury Severity Scoring (TRISS) method and compare the outcome with the registry data from the Major Trauma Outcome Study (MTOS).Material and Method: A...

Soffer, A.; Myers, J.A., 1968: The Jay Arthur Myers dedication issue. Diseases of the Chest 53(6): 665-669

Halder, G.E.; Bearman, G.; Sanogo, K.; Stevens, M.P., 2014: Water sanitation, access, use and self-reported diarrheal disease in rural Honduras. Only 79% of individuals living in rural Honduras use improved water sources. Inadequate drinking water quality is related to diarrheal illness, which in Honduras contributes to 18.6 episodes of diarrhea per child year in children under five years...

Fu, Y.; Sun, Q.; Liu, B.; Xu, H.; Wang, Y.; Zhu, W.; Pan, L.; Zhu, L., 2016: Epidemiological characteristics and pathogens attributable to hand, foot, and mouth disease in Shanghai, 2008-2013. Hand, foot, and mouth disease (HFMD) is a common childhood illness caused by enteroviruses. A passive surveillance system has been implemented in Shanghai Pudong since 2008 and etiology surveillance since 2009.We characterized the epidemiology and...

Gray, N., 1979: Will grey partridges survive?. Game Conservancy Annual Review, 1978: 48-52

Koide, A.; Bailey, C.W.; Huang, X.; Koide, S., 1998: The fibronectin type III domain as a scaffold for novel binding proteins. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The beta-sandwich structure of FN3 closely resembles that of immunoglobulin domains. We have prepared a phag...

Bauer, A.M.; Azzone, V.; Alexander, L.; Goldman, H.H.; Unützer, Jürgen.; Frank, R.G., 2012: Are patient characteristics associated with quality of depression care and outcomes in collaborative care programs for depression?. To determine whether demographic or clinical characteristics of primary care patients are associated with depression treatment quality and outcomes within a collaborative care model. Collaborative depression care, based on principles from the Impr...

Jensen, T.E.; Leutert, R.; Rasmussen, Søren.T.; Mouatt, J.R.; Christiansen, M.L.B.; Jensen, B.R.; Richter, E.A., 2012: EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle. In mice, certain proteins show a highly confined expression in specific muscle groups. Also, resting and exercise/contraction-induced phosphorylation responses are higher in rat skeletal muscle with low mitochondrial content compared to muscles wi...