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Cloning and expression of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang)


, : Cloning and expression of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang). Journal of Agricultural and Food Chemistry 48(7): 3052-3057

Pectin methylesterase (PME) is the key enzyme responsible for the gelation of jelly curd in the water extract of jelly fig (Ficus awkeotasang) achenes. The jelly fig PME extracted from achenes was isoelectrofocused at pH 2.5 and subjected to N-terminal amino acid sequencing. A cDNA fragment encoding the mature protein of this acidic PME was obtained by PCR cloning using a poly(T) primer and a degenerate primer designed according to the N-terminal sequence of the purified PME. The complete cDNA sequence of its precursor protein was further obtained by PCR using the same strategy. The PME clone was overexpressed in Escherichia coli, and its expressed protein was immunologically recognized as strongly as the original antigen using antibodies against purified PME. Fractionation analysis revealed that the overexpressed PME was predominantly present in the pellet and thus presumably formed insoluble inclusion bodies in E. coli cells.

US$19.90

PMID: 10898664

DOI: 10.1021/jf000273d


Other references

Tone, Y.; Kidani, Y.; Ogawa, C.; Yamamoto, K.; Tsuda, M.; Peter, C.; Waldmann, H.; Tone, M., 2014: Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Glucocorticoid-induced TNFR (Gitr) and Ox40, two members of the TNFR superfamily, play important roles in regulating activities of effector and regulatory T cells (Treg). Their gene expression is induced by T cell activation and further upregulate...

Koeger B.; Horak I., 1987: Isolation of novel human retrovirus related sequences by hybridization to synthetic oligonucleotides complementary to the proline transfer rna primer binding site. Synthetic oligonucleotides complementary to putative retroviral primer-binding sites were used as hybridization probes to detect novel retroviruslike sequences. An 8.1-kilobase element with structural features of a retroviral provirus was isolated...

Froget, Joseph, 1960: Modern orientation of genetic studies. A brief historical review of the trend of research in genetics, classified into formal, biochemical and population varieties.

Swietojanska, J., 2006: A new species of Notosacantha Chevrolat from Halmahera, Indonesia Coleoptera Chrysomelidae Cassidinae. Notosacantha halmaherana, new to the science, is described from Halmahera, Moluccas.

Woods,L.C.,III.T.eisen,D.D.; He,S.Y., 2008: Efficacy of Aqui-S as an anesthetic for market-sized striped bass. Market-sized 2-year-old striped bass <i>Morone saxatilis</i> (average weight, 645 g) were evaluated in three different concentrations of Aqui-S at one of three different temperatures. Time to stage 4 of anesthesia, recovery time, and p...

Griffiths, HI., 1992: Police prosecutions and badger persecution in England and Wales. Animal Welfare, 14: 291-296

Kivilcim, M.; Peyman, G.A.; El-Dessouky, E.S.; Kazi, A.A.; Cheema, R.; Hegazy, H., 2000: Retinal toxicity of triamcinolone acetonide in silicone-filled eyes. To determine the retinal toxicity of triamcinolone acetonide at different doses in vitrectomized, silicone-filled rabbit eyes. Vitrectomy with silicone oil placement was performed in 32 rabbit eyes. A dosage of 1 mg/0.025 mL, 2 mg/0.05 mL, or 4 mg...

Subbarao V.V., 1981: Effect of photo deprivation and androgen on male reproductive organs. Israel Journal of Medical Sciences 17(8): 729-730

Teschke R.; Hasumura Y.; Lieber C.S., 1974: Hepatic microsomal ethanol oxidizing system its catalase independent nature. Federation Proceedings 33(3 PART 1): 538

Kormilev, N.A.II, 1938: Beitrag zur Kenntnis der Verbreitung jugoslavischer Hemiptera-Heteroptera (Serbien und Sudserbien). BullSci. Skoplie: 167-172