geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of conditioned medium on activities of PAL, CHS, DAHP synthase (DS-Co and DS-Mn) and anthocyanin production in suspension cultures of Fragaria ananassa


, : Effects of conditioned medium on activities of PAL, CHS, DAHP synthase (DS-Co and DS-Mn) and anthocyanin production in suspension cultures of Fragaria ananassa. Plant Science 160(2): 355-360

A conditioned medium (CM) prepared from cell suspension cultures of strawberry stimulated anthocyanin synthesis. The effect was significantly (P<0.05) greater than that of synthetic medium (SM), with macronutrient concentrations, carbohydrate concentrations and pH adjusted to those of CM. The activity of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase (EC 4.1.2.15) (DS-Mn, DS-Co), phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and chalcone synthase (CHS, EC 2.3.1.74) were monitored in the CM- and SM-cultured cells. PAL and CHS activities were found to increase significantly (P<0.05) in the CM-cultured cells. CHS transcript levels were higher in the CM-cultured cells compared to transcript abundance in SM-cultured cells. There was no significant difference in the DS-Mn and DS-Co activities of cells grown in conditioned or synthetic media.

US$19.90

PMID: 11164607

DOI: 10.1016/s0168-9452(00)00399-x


Other references

Rao M.V.; Gedam P.H., 1985: Observations on component fatty acids of indian sardine oil and its upgraded samples. Three samples of sardine oil, 1 obtained by extraction of the whole body of sardine fish and the others 2 by upgrading of the former by different methods, were analyzed using chromatographic techniques for their fatty acid compositions. The fatty...

Hull, C.J.; Storrs, J.A., 1973: Data collection in the labour ward: a new approach. British Journal of Anaesthesia 45(12): 1236-1237

Debry, R.W., 2005: The systematic component of phylogenetic error as a function of taxonomic sampling under parsimony. The effect of taxonomic sampling on phylogenetic accuracy under parsimony is examined by simulating nucleotide sequence evolution. Random error is minimized by using very large numbers of simulated characters. This allows estimation of the consist...

Barth, R., 1958: Estmulos qumicos como meio de comunicaYao entre os sexos em Lepidopteros. An bras. Cienc, Rio de: 343-362

Birch, P.R.J.; Armstrong, M.; Bos, J.; Boevink, P.; Gilroy, E.M.; Taylor, R.M.; Wawra, S.; Pritchard, L.; Conti, L.; Ewan, R.; Whisson, S.C.; van West, P.; Sadanandom, A.; Kamoun, S., 2009: Towards understanding the virulence functions of RXLR effectors of the oomycete plant pathogen Phytophthora infestans. Plant pathogens establish infection by secretion of effector proteins that may be delivered inside host cells to manipulate innate immunity. It is increasingly apparent that the ubiquitin proteasome system (UPS) contributes significantly to the re...

Robert, J.A.; Braun, S.; Leontic, E.; Chamorro Spikin, G.; Casanegra, P.; Giesen, E., 1985: Risk, maternal and fetal of pregnancy in patients with heart valve diseases. Revista Chilena de Obstetricia Y Ginecologia 50(3): 175-183

Spratt, T.E.; Campbell, C.R., 1994: Solvent deuterium isotope in the reaction of O-6-alkylguanine-DNA alkyltransferase with oligodeoxynucleotides containing O-6-methylguanine. Proceedings of the American Association for Cancer Research Annual Meeting 35(0): 537

Matsumoto, Yoshiyuki, 2000: Delayed damage after radiation therapy for head and neck cancer. I investigated radiation damage, including osteoradionecrosis, arising from tooth extraction in fields that had received radiation therapy for head and neck cancer, and evaluated the effectiveness of pilocarpine for xerostomia. Between January 199...

Urbain, R.; Michel, J.B.; Bouveret, P.; Wisnewsky, C.; Schwartz, K.; Mercadier, J.J., 1989: Left ventricular accumulation of messenger ribonucleic acid coding for the natriuretic atrial factor in various experimental models of cardiac hypertrophy in rats. Cardiac hypertrophy secondary to chronic hemodynamic overload is associated with an increase in the ventricular concentration of the messenger ribonucleic acid (mRNA) coding for the atrial natriuretic factor (ANF). We have compared, in male Wistar...

DeLong Stephen B.; Pelletier Jon D.; Arnold Lee, 2007: Bedrock landscape development modeling; calibration using field study, geochronology, and digital elevation model analysis. Stream power-based models of bedrock landscape development are effective at producing synthetic topography with realistic fluvial-network topology and three-dimensional topography, but they are difficult to calibrate. This paper examines ways in w...