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Enzymatic determination of acetylcarnitine for diagnostic applications

, : Enzymatic determination of acetylcarnitine for diagnostic applications. Journal of Pharmaceutical and Biomedical Analysis 24(5/6): 1147-1150

An enzymatic method was proposed for the determination of acetylcarnitine (AcCar), even when carnitine (Car), non-acetylated form, co-exists. The method consists of 4 enzymatic reactions: first, AcCar is hydrolysed by acylcarnitine hydrolase to yield acetate; followed by the other 3 reactions coupled with 3 enzymes (acetate kinase, pyruvate kinase and lactate dehydrogenase); finally, the acetate formation causes a decrease in NADH.


PMID: 11248512

DOI: 10.1016/s0731-7085(00)00575-6

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