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Isolation of mesophyll protoplasts of Cucumis spp. and Cucurbita spp

, : Isolation of mesophyll protoplasts of Cucumis spp. and Cucurbita spp. Acta Horticulturae ( 510): 425-431

Well-developed first or second true leaves, originating from in vitro plants of two Cucumis species (C. melo, C. metuliferus) and three Cucurbita species (C. ficifolia, C. maxima, C. pepo), were used for protoplast isolation. The optimal composition of the enzyme solution and the most suitable isolation method were studied. The density and viability of protoplasts were used as efficiency criteria.


DOI: 10.17660/ActaHortic.2000.510.68

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