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Modification of the reactivity of spinach chloroplast thioredoxin f by site-directed mutagenesis

, : Modification of the reactivity of spinach chloroplast thioredoxin f by site-directed mutagenesis. Plant Science Limerick 149(2): 183-190

Spinach chloroplast thioredoxin f has a third cysteine residue which is surface exposed and close to the active site disulfide. In addition its N-terminus is rather long compared to other thioredoxins. By site-directed mutagenesis the third cysteine has been replaced, the long N-terminal tail has been removed and the properties of the modified proteins have been examined.


DOI: 10.1016/s0168-9452(99)00168-5

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