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Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis

, : Multiplex polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis. Journal of Dairy Science 84(5): 1140-1148

To improve diagnosis of mastitis in dairy cattle, a multiplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of the four major bacterial causes of bovine mastitis, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis. The target sequence was the 16S to 23S rRNA spacer regions. The performance of the assay was examined with 117 milk samples collected from a subclinically infected herd, and the diagnostic specificities and sensitivities of the multiplex PCR were compared with conventional culture. PCR was significantly more sensitive than culture for detection of S. aureus and S. uberis, but there were no significant differences in sensitivities between PCR and culture for the detection of S. agalactiae and S. dysgalactiae. The results suggest that this multiplex PCR assay could be used as an alternative method in routine diagnosis for rapid, sensitive, and specific simultaneous detection of S. aureus, S. agalactiae, S. dysgalactiae, and S. uberis in milk samples.


PMID: 11384040

DOI: 10.3168/jds.S0022-0302(01)74574-2

Other references

Harned, R.W., 1915: Spraying Peaches and Plums. Brown rot and plum curculio (Conotrachelus nenuphar), on peaches and plums, are best controlled by the use of self-boiled lime-sulphur mixture and arsenate of lead. The lime-sulphur is prepared as follows: 81b. fresh lime are covered with cold wat...

Bochantsev V.P., 1986: Irania new section of the genus salsola chenopodiaceae. Botanicheskii Zhurnal (St Petersburg) 71(10): 1400-1401

Jackson, D.R.; Corrier, D.E.; Pillai, S.D.; Woodward, C.L.; Pena, J.; Ricke, S.C., 1999: Gene amplification approach for detection of Salmonella typhimurium in poultry aerosol samples. Poultry Science 78(SUPPL 1): 58-59

Mason Brian; Wiik H.B., 1961: The composition of the Ottawa, Chateau-Renard, Mocs, and New Concord meteorites. American Museum Novitates 2069: 1-25

Campanha V.P.; Futuro Neto H.A.; Pires J.G.P., 1977: Bufeniode an anti hypertensive agent with alpha adrenergic and ganglionic blocking properties. Revista Brasileira de Pesquisas Medicas e Biologicas 10(6): 444

Zaverukha, F.M., 1952: Nicotinic acid in glaucoma. Vestnik Oftalmologii 31(1): 31-35

Stanton, J.B.; Knowles, D.P.; O'Rourke, K.I.; Herrmann-Hoesing, L.M.; Mathison, B.A.; Baszler, T.V., 2008: Small-ruminant lentivirus enhances PrPSc accumulation in cultured sheep microglial cells. Sheep scrapie is the prototypical transmissible spongiform encephalopathy (prion disease), which has a fundamental pathogenesis involving conversion of normal cellular prion protein (PrP<sup>C</sup> [C superscript stands for cellular])...

Hall, B.P., 1952: A new race of Agapornis from Angola. Bulletin of the British Ornithologists' Club, 72: 25

Lesiuk, H.; Barnes, K.; Tam, A.; Todd, M., 1995: Post-ischemic administration of trans-ACPD is protective against ischemic neuronal injury in rodents. Stroke 26(1): 166

Abraham F.A.; Teller J.; Nissenkorn I.; Ben Sira I., 1989: Long term electrophysiological evaluation of cryotherapy for active stage of retinopathy of prematurity. Investigative Ophthalmology & Visual Science 30(3 SUPPL): 317