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Seed germination enhancement in Satureja montana L. ssp. montana

, : Seed germination enhancement in Satureja montana L. ssp. montana. Seed Science and Technology 29(2): 347-355

To enhance seed germination, seeds were subjected to : (i) rupture of the coat by piercing, scarification with concentrated H2SO4 (exposure for 1, 5, 10, 15 and 20 minutes) or sandpaper (P 180), ultrasound, dry (dry, hot air at 80, 100, 110 or 120 degrees C (+or-2 degrees C) for 1, 2 or 3 minutes) and wet (A: dipping in boiling water for 1, 2 or 3 minutes and then cooled in distilled water; B: dipped in 100 ml of hot water (90 or 70 degrees C) and then left to cool at 20-22 degrees C) heat; (ii) softening of the coat or removal of possible physiological dormancy by pre-chilling at 5-7 degrees C for 60 days, soaking in water for 24 and 48 h, in ethanol for 8 h and in sodium hypochlorite for various times (5, 10, 30, 60, 90, 120 and 180 minutes); (iii) embryo stimulation with GA3 (50 micro g/litre and 500 micro g/litre). The only effective treatments were seed coat piercing, sand paper scarification (final germination of 82-85% and a germination rate of 72-74) and to a lesser extent prechilling. Acid scarification was ineffective if performed for one minute and harmful if performed for 5 minutes or more. All the heat treatments, the soaking in 70% ethanol and leaving the seeds to germinate in 1-4% ethanol-water solutions were harmful to the seeds. Soaking in sodium hypochlorite for 5 minutes had no effect, while soaking for 10 minutes or more was harmful. Ultrasound treatment had no effect if applied for 30 minutes and was harmful if applied for 60 minutes. Soaking in water and the addition of GA3 were ineffective.


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