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Structure - function relationship of extensin-like cotton proteins

, : Structure - function relationship of extensin-like cotton proteins. Chemistry of Natural Compounds . 2001; 36(4): 404-407

The effect of deglycosylation on cotton extensin-like proteins (ELP) in a tissue culture was studied to determine structure-function relationship in ELP. Cotton ELP were deglycosylated by anhydrous HF. The biological activity of the deglycosylated ELP (d-ELP) was determined using KML murine melanoma cell line and live cell count. ELP and d-ELP (100 micro g/ml) were added to cells in nutrient medium after 24 h. 3H-thymidine was then introduced for 1 h and live cells were counted.


DOI: 10.1023/A:1002861301751

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