geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Tissue-specific subcellular immunolocalization of a myosin-like protein in maize root apices


, : Tissue-specific subcellular immunolocalization of a myosin-like protein in maize root apices. Protoplasma 212(3-4): 137-145

Using a heterologous myosin antibody raised against the whole molecule of bovine muscle myosin, we have identified a myosin-like protein in maize. Immunoblots of subcellular fractions isolated from roots identified one distinct band at about 210 kDa in the microsomal protein fraction and one band at about 180 kDa in the soluble protein fraction. Indirect immunofluorescence was performed using maize root apex sections to reveal endocellular distributions of the myosin-like protein. Both diffuse and particulate labelling patterns were observed throughout the cytoplasm of all root cells. In mitotic cells, myosin-like protein was excluded from spindle regions. Amyloplast surfaces were labelled prominently in cells of the root cap statenchyma and in all root cortex cells. On the other hand, myosin-like protein was prominently enriched at cellular peripheries in cells of the pericycle and outer stele in the form of continuous peripheral labelling. From all root apex tissues, phloem elements showed the most abundant presence of myosin-like protein.

US$19.90

DOI: 10.1007/bf01282915


Other references

Saxena, V.K.; Sharma, R.N., 1999: Antimicrobial activity of the essential oil of Toddalia asiatica. The essential oil from the leaves was active against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella newport, Staphylococcus aureus, Aspergillus fumigatus, A. niger, Microspermum gypsum [Microsporum gypseum] and Rhizopu...

Korochkin, L.; Konyukhov, B., 1988: Genetics of animal development. Soviet genetics 23(10): 1233-1240

Haley, S.J.; Barnes, J., 2017: New York Community Health Centers' Population Health Activities: Findings from a Statewide Assessment. The U.S. spends just 5% of its health care budget to prevent morbidity and mortality. This study surveyed N.Y. State community health centers' (CHCs) population health activities aligned with the N.Y. Prevention Agenda (response rate of 72%)....

van Loon, JJA. van Eeuwijk, FA., 1989: Chemoreception of amino acids in larvae of two species of Pieris. Specificity and sensitivity of gustatory neurons in response to 22 amino acids were studied in larvae of Pieris brassicae and P. rapae using electrophysiological methods. Twelve amino acids stimulated a specific amino acid receptor cell in the lat...

Sawyer, R.; Lawler, A.; Overstreet, R., 1975: Marine leeches of the eastern United States and the Gulf of Mexico with a key to the species. Key to marine and estuarine leeches of eastern United States and Gulf of Mexico, diagnoses, synonymy, host and geographical distribution, ecology

Basu, B.D., 1896: Some Observations on the Proper Cooking of Dals (Indian Pulses). Indian Medical Gazette 31(12): 437-438

Stefanescu, C.; Tamas, M.; Barbu, L., 2006: Anatomical studies on Scopolia carniolica Jacq. vegetative organs. The structures of vegetative organs and the biometrical parameters of the leaf blade of S. carniolica were studied. Rhizome cross-sections showed a secondary structure with strongly developed parenchyma elements. The xylem vessels were rare and ar...

Chowchuen, B.; Godfrey, K., 2004: Development of a network system for the care of patients with cleft lip and palate in Thailand. Cleft lip and palate are major public health concerns in Thailand, particularly in the north-east. The challenge for a developing country is to adapt to its unique environment the form of multidisciplinary care of patients with clefts in industria...

Serratto, M.; Benvenuto, R., 1959: Notes on chlorothiazide. Gazzetta Medica Italiana 118: 342-347

Wenchel H M.; Kretzschmar H J., 1987: Diminution of the ethylene oxide residues in medical synthetic material after sterilization by overpressure procedure. After an EO-sterilization by an overpressure procedure (1.7 bar, 45.degree. C) the EO-rest concentrations with PVC-tubes and angiographycatheters were determined within the material and in the air directly above the surface of the material. Withou...