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A modified hepatitis B virus core particle containing multiple epitopes of the Plasmodium falciparum circumsporozoite protein provides a highly immunogenic malaria vaccine in preclinical analyses in rodent and primate hosts


, : A modified hepatitis B virus core particle containing multiple epitopes of the Plasmodium falciparum circumsporozoite protein provides a highly immunogenic malaria vaccine in preclinical analyses in rodent and primate hosts. Infection and Immunity 70(12): 6860-6870

Despite extensive public health efforts, there are presently 200 to 400 million malaria infections and 1 to 2 million deaths each year due to the Plasmodium parasite. A prime target for malaria vaccine development is the circumsporozoite (CS) protein, which is expressed on the extracellular sporozoite and the intracellular hepatic stages of the parasite. Previous studies in rodent malaria models have shown that CS repeat B-cell epitopes expressed in a recombinant hepatitis B virus core (HBc) protein can elicit protective immunity. To design a vaccine for human use, a series of recombinant HBc proteins containing epitopes of Plasmodium falciparum CS protein were assayed for immunogenicity in mice [A. Birkett, B. Thornton, D. Milich, G. A. Oliveira, A. Siddique, R. Nussenzweig, J. M. Calvo-Calle, and E. H. Nardin, abstract from the 50th Annual Meeting of the American Society of Tropical Medicine and Hygiene 2001, Am. J. Trop. Med. Hyg. 65(Suppl. 3):258, 2001; D. R. Milich, J. Hughes, J. Jones, M. Sallberg, and T. R. Phillips, Vaccine 20:771-788, 2001]. The present paper summarizes preclinical analyses of the optimal P. falciparum HBc vaccine candidate, termed ICC-1132, which contains T- and B-cell epitopes from the repeat region and a universal T-cell epitope from the C terminus of the CS protein. The vaccine was highly immunogenic in mice and in Macaca fascicularis (cynomolgus) monkeys. When formulated in adjuvants suitable for human use, the vaccine elicited antisporozoite antibody titers that were logs higher than those obtained in previous studies. Human malaria-specific CD4(+)-T-cell clones and T cells of ICC-1132-immunized mice specifically recognized malaria T-cell epitopes contained in the vaccine. In addition to inducing strong malaria-specific immune responses in naïve hosts, ICC-1132 elicited potent anamnestic antibody responses in mice primed with P. falciparum sporozoites, suggesting potential efficacy in enhancing the sporozoite-primed immune responses of individuals living in areas where malaria is endemic.

US$19.90

PMID: 12438363

DOI: 10.1128/IAI.70.12.6860-6870.2002


Other references

Gambuzzi, E.L.; Zehnder, R.; Castignani, H.; Chimicz, J., 2005: Analysis of dairy farms in Argentina. 1. Principal variables affecting productivity. Revista Argentina de Produccion Animal 25(Supl. 1): SP35

Aravind, L.; Koonin, E.V., 1999: Novel predicted RNA-binding domains associated with the translation machinery. Two previously undetected domains were identified in a variety of RNA-binding proteins, particularly RNA-modifying enzymes, using methods for sequence profile analysis. A small domain consisting of 60-65 amino acid residues was detected in the rib...

Dadamukhamedov, A.N., 1977: Influence of suppurative inflammation of the middle ear on the course of tuberculous meningitis. Problemy Tuberkuleza: 54-57

Butler, R.D.W., 1947: The relationship between the ophthalmic surgeon and the orthoptist. British Orthoptic Journal 4(4): 105-105

Zil'bergleit S.; Goryshin I.Yu; Lantsov V.A., 1990: Transposition and immunity in composite transposons analysis of recombinant elements of tn5 with modified activity of transposase and repressor. Data were presented on the structure of 5 recombinant transposons and on the effectiveness of transposition and the degree of immunity. It is of interest that there was no correlation between changes in transposase and repressor functions caused b...

Detre C., 1974: What does the concept pelsonian designate. Foldtani Kozlony 104(3): 336-340

Saburomaru T.; Tsukahara H., 1984: The life history of girella punctata on the northern coast of fukuoka japan. The life history of G. punctata (Girellidae) was studied at the northern coast of Fukuoka from 1975-1977. As the subjects of this study, 0+ and 1+ age fishes of < 150 mm in BL [body length] were investigated. To clarify the life history of this...

Haken, H., 1992: Synergetics of the brain An outline of some basic ideas. Basar, E and T H Bullock Brain Dynamics Series: Induced rhythms in the brain xix+483p Birkhaeuser Boston: Cambridge, Massachusetts, USA; Basel, Switzerland Illus ISBN 0-8176-3537-8; ISBN 3-7643-3537-8 : 417-421

Meshkov, T.; Shinkov, D.; Eshkenazi, M., 1981: Biphasic peroxidase in the study of Fc receptors of leukemic lymphocytes. The authors examined Fc receptors of cytoplasmic membrane by means of a two-stage immunoperoxidase technique: at the first stage the immuno-gamma-globulins (IgG), labelled with peroxidase, were connected with the membrane receptors, but at the sec...

Jones H.W.Jr; Garcia S.C.; Klingensmith G.J., 1977: Necessity for and the technique of secondary surgical treatment of the masculinized external genitalia of patients with virilizing adrenal hyperplasia. Lee, Peter A Et Al (Ed ) Congenital Adrenal Hyperplasia Symposium Baltimore, Md , Usa, Oct 20-24, 1975 Xxii+532p Illus University Park Press: Baltimore, Md , Usa; London, England Isbn 0-8391-0974-1 347-352