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IR spectra of cytochrome c denatured with deuterated guanidine hydrochloride show increase in beta sheet

, : IR spectra of cytochrome c denatured with deuterated guanidine hydrochloride show increase in beta sheet. Biopolymers 72(3): 193-204

Attenuated total reflectance Fourier transform IR (ATR-FTIR) spectra are obtained for horse heart ferricytochrome c in solutions of 0-7 M guanidine hydrochloride and deuterated guanidine hydrochloride. Substitutions of deuterium for hydrogen in both the denaturant and protein provide resolvable amide I spectra over a wide range of denaturant concentrations.


PMID: 12722115

DOI: 10.1002/bip.10337

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