+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

IRES-driven translation is stimulated separately by the FMDV 3'-NCR and poly(A) sequences

, : IRES-driven translation is stimulated separately by the FMDV 3'-NCR and poly(A) sequences. Nucleic Acids Research 30(20): 4398-4405

The 3' end region of foot-and-mouth disease virus (FMDV) consists of two distinct elements, a 90 nt untranslated region (3'-NCR) and a poly(A) tract. Removal of either the poly(A) tract or both the 3'-NCR and the poly(A) tract abrogated infectivity in susceptible cells in the context of a full-length cDNA clone. We have addressed the question of whether the impairment of RNA infectivity is related to defects at the translation level using a double approach. First, compared to the full-length viral RNA, removal of the 3' sequences reduced the efficiency of translation in vitro. Secondly, a stimulatory effect of the 3' end sequences on IRES-dependent translation was found in vivo using bicistronic constructs. RNAs carrying the FMDV 3' end sequences linked to the second cistron showed a significant stimulation of IRES-dependent translation, whereas cap-dependent translation was not affected. Remarkably, IRES-dependent stimulation exerted by the poly(A) tract or the 3'-NCR seems to be the result of two separate events, as the 3'-NCR alone enhanced IRES activity on its own. Under conditions of FMDV Lb protease-induced translation shut-off, the stimulation of IRES activity reached values above 6-fold in living cells. A northern blot analysis indicated that IRES stimulation was not the consequence of a change in the stability of the bicistronic RNA produced in transfected cells. Analysis of the RNA-binding proteins interacting with a mixture of 3' end and IRES probes showed an additive pattern. Altogether, our results strongly suggest that individual signals in the viral 3' end ensure stimulation of FMDV translation.


PMID: 12384586

DOI: 10.1093/nar/gkf569

Other references

Kishki, I.G., 1969: Peculiarities of fine structure and virus reproduction. Mikrobiolohichnyi Zhurnal 31(2): 141-146

Lopater, S.; Hahn, J.F., 1975: One- and two-frequency stimulation of tactile receptors: test of a model. Journal of the Acoustical Society of America 57(3): 696-701

Chong, S.F.; Lou, Q.; Mason, T.; Leland, D.; Lee, C.H.; Fitzgerald, J.F., 1994: Intercurrent cytomegalovirus and other viral infections are not a major cause of exacerbations in chronic inflammatory bowel disease in childhood. Gastroenterology 106(4 SUPPL ): A664

Grant, B.; Dufour, M.S.inson, F.T.wle, L.; Bertolucci, D., 1987: Proposed coding of alcoholss role in casualties. Alcohol health and research world National Institute on Alcohol Abuse and Alcoholism 12(1): 48-50

Rorie R.W.; Pendleton R.J.; Pool S.H.; Youngs C.R.; Godke R.A., 1986: The viability of bovine half embryos produced before and after liquid nitrogen freezing. Journal of In Vitro Fertilization & Embryo Transfer 3(2): 66

Martinson, V.A., 1975: Cytological studies of diploid and tetraploid Theobroma cacao. Bivalent formation was observed at meiosis in the diploid, and although there was a low apparent chiasma frequency, disjunction was regular. In the two colchicine-induced autotetraploids studied, there was a high frequency of bivalents compared wi...

Dahl, T.W., 2016: GEOCHEMISTRY. Identifying remnants of early Earth. Science 352(6287): 768-769

Ehrlich, B.E.; Schen, C.R.; Spudich, J.L., 1984: Bacterial rhodopsins monitored with fluorescent dyes in vesicles and in vivo. Three retinal-containing pigments have been detected in Halobacterium halobium membranes: bacteriorhodopsin (bR), halorhodopsin (hR), and slow-cycling rhodopsin (sR). The first two hyperpolarize the cell membrane by electrogenic transport of H+ an...

Péan, J-Emile., 2016: Personalities in the history of medicine. Jules-Emile Péan. Praxis 105(7): 422-422

MacLaren, R.; Campbell, J., 2014: The authors reply. Critical Care Medicine 42(9): E637-E638