+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

ISSR-PCR technique: a useful method for characterizing new allotetraploid somatic hybrids of mandarin

, : ISSR-PCR technique: a useful method for characterizing new allotetraploid somatic hybrids of mandarin. Plant Cell Reports 20(12): 1162-1166

Somatic hybrids between Redblush and Duncan grapefruit (Citrus paradisi Macfadyen) with Avana, Tardivo di Ciaculli mandarin (C. deliciosa Tenore) and Fortune mandarin (C. reticulata Blanco) were obtained by symmetric protoplast fusion. Isozyme banding pattern analysis and flow cytometry were used for early screening. DNA of the hybrids was then extracted and subjected to inter-simple sequence repeat polymerase chain reaction (ISSR-PCR) using different primers anchored at the 5 and 3 ends. PCR products were resolved on 1.5% agarose gels and stained with ethidium bromide. All the bands from the parental cultivars were present in the somatic hybrids. The usefulness and efficiency of the ISSR-PCR method as a screening technique for Citrus somatic hybrids are discussed.


DOI: 10.1007/s00299-002-0450-3

Other references

Rausch Stroomann J G.; Miehlke K.; Wirth B.; Thomas E.; Petry R., 1969: Mode of action of intra muscular and intra articular injections of depot steroids measured by cortisol production in men. Anon Abstracta Xii Congressus Rheumatologicus Internationalis Praga (Abstracts 12th International Rheumatological Congress ) Np J R Geigy S A : basle, Switz 402

Daniels, K.; Haddow, K., 2007: Otophyma: a case report. Objective: We report a rare case of otophyma.Method: A case report of otophyma and a review of the current literature concerning otophyma and the more common rhinophyma, are presented.Results: A 46-year-old male presented with slow growing fleshy...

Lal H.; Yaden S.; Forster M.J., 1990: Elevation of blood pressure as the basis for discriminative stimuli produced by methoxamine. Male, hooded rats were trained to detect methoxamine using a discrimination paradigm in which responding under an FR10 schedule was reinforced on one lever following an injection of methoxamine (1.25 mg/kg) and on an alternate lever following an i...

Sumarokov, A.V., 1959: Cardiac arrhythmia in neuroses. Sovetskaia Meditsina 23: 19-29

Golychev, V.N., 1989: Honey in the treatment of cataract. Fel'dsher i Akusherka 54(4): 57-58

Kowalsky, S.F.; Echols, R.M.; Peck, F.; Lesar, T.S.; Schwartz, M.T., 1986: Second-generation antibiotic-order sheet. American Journal of Hospital Pharmacy 43(5): 1182, 1184-1182, 1184

Graham, D.Y.; Kato, M.; Asaka, M., 2008: Gastric endoscopy in the 21st century: appropriate use of an invasive procedure in the era of non-invasive testing. Background. The acceptance of the premise that Helicobacter pylori infection is aetiologically related to gastric cancer and peptic ulcer and that the risk of gastric cancer among Helicobacter pylori infected individuals is related to the extent,...

Kolpakov V.V.; Kind N.V., 1974: Siberian areas along the Lena River. 3: 230-238 1974

Gitelman, S.E.; Feldman, B.J.; Rosenthal, S.M., 2006: Nephrogenic syndrome of inappropriate antidiuresis: a novel disorder in water balance in pediatric patients. The syndrome of inappropriate antidiuretic hormone secretion (SIADH) is a common cause of hyponatremia. We report findings in 2 unrelated male infants whose clinical presentation and laboratory findings were consistent with SIADH, but who exhibite...

García Borda, F.J.; Gutiérrez Calvo, A.; Nuño Vázquez-Garza, J.; López Fernández, J.I.; Pascual Montero, J.A.; Lucea Martínez, C., 1988: Primary lymphoma of the stomach. Revista Quirurgica Espanola 15(5): 219-222